Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Anaerobic methane oxidation coupled to nitrite reduction can be a potential methane sink in coastal environments
Ist Teil von
Applied microbiology and biotechnology, 2016-08, Vol.100 (16), p.7171-7180
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2016
Link zum Volltext
Quelle
SpringerLink (Online service)
Beschreibungen/Notizen
In the current study, we investigated nitrite-dependent anaerobic methane oxidation (N-DAMO) as a potential methane sink in the Hangzhou Bay and the adjacent Zhoushan sea area. The potential activity of the N-DAMO process was primarily observed in Hangzhou Bay by means of
13
C-labeling experiments, whereas very low or no potential N-DAMO activity could be detected in the Zhoushan sea area. The measured potential N-DAMO rates ranged from 0.2 to 1.3 nmol
13
CO
2
g
−1
(dry sediment) day
−1
, and the N-DAMO potentially contributed 2.0–9.4 % to the total microbial methane oxidation in the examined sediments. This indicated that the N-DAMO process may be an alternative pathway in the coastal methane cycle. Phylogenetic analyses confirmed the presence of
Candidatus
Methylomirabilis oxyfera
-like bacteria in all the examined sediments, while the group A members (the dominant bacteria responsible for N-DAMO) were found mainly in Hangzhou Bay. Quantitative PCR showed that the 16S rRNA gene abundance of
Candidatus M. oxyfera
-like bacteria varied from 5.4 × 10
6
to 5.0 × 10
7
copies g
−1
(dry sediment), with a higher abundance observed in Hangzhou Bay. In addition, the overlying water NO
3
−
concentration and salinity were identified as the most important factors influencing the abundance and potential activity of
Candidatus M. oxyfera
-like bacteria in the examined sediments. This study showed the evidence of N-DAMO in coastal environments and indicated the importance of N-DAMO as a potential methane sink in coastal environments.