Details

Autor(en) / Beteiligte

• Li, Wenzhe
• Yu, Rui
• Ma, Biao
• Yang, Yan
• Jiao, Xinyan
• Liu, Yang
• Cao, Hongyu
• Dong, Weijie
• Liu, Linhua
• Ma, Keli
• Fukuda, Tomohiko
• Liu, Qingping
• Ma, Tonghui
• Wang, Zhongfu
• Gu, Jianguo
• Zhang, Jianing
• Taniguchi, Naoyuki

Titel

Core fucosylation of IgG B cell receptor is required for antigen recognition and antibody production

Ist Teil von

• The Journal of immunology (1950), 2015-03, Vol.194 (6), p.2596-2606

Ort / Verlag

United States

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Ag recognition and Ab production in B cells are major components of the humoral immune response. In the current study, we found that the core fucosylation catalyzed by α1,6-fucosyltransferase (Fut8) was required for the Ag recognition of BCR and the subsequent signal transduction. Moreover, compared with the 3-83 B cells, the coalescing of lipid rafts and Ag-BCR endocytosis were substantially reduced in Fut8-knockdown (3-83-KD) cells with p31 stimulation and then completely restored by reintroduction of the Fut8 gene to the 3-83-KD cells. Indeed, Fut8-null (Fut8(-/-)) mice evoked a low immune response following OVA immunization. Also, the frequency of IgG-producing cells was significantly reduced in the Fut8(-/-) spleen following OVA immunization. Our results clearly suggest an unexpected mode of BCR function, in which the core fucosylation of IgG-BCR mediates Ag recognition and, concomitantly, cell signal transduction via BCR and Ab production.