UNIVERSI
TÄ
TS-
BIBLIOTHEK
P
ADERBORN
Anmelden
Menü
Menü
Start
Hilfe
Blog
Weitere Dienste
Neuerwerbungslisten
Fachsystematik Bücher
Erwerbungsvorschlag
Bestellung aus dem Magazin
Fernleihe
Einstellungen
Sprache
Deutsch
Deutsch
Englisch
Farbschema
Hell
Dunkel
Automatisch
Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist
gegebenenfalls
nur via VPN oder Shibboleth (DFN-AAI) möglich.
mehr Informationen...
Universitätsbibliothek
Katalog
Suche
Details
Zur Ergebnisliste
Ergebnis 26 von 92
Datensatz exportieren als...
BibTeX
Priming Endothelial Cells With a Melanoma-Derived Extracellular Matrix Triggers the Activation of αvβ3/VEGFR2 Axis
Journal of cellular physiology, 2016-11, Vol.231 (11), p.2464-2473
Helal-Neto, Edward
Brandão-Costa, Renata M.
Saldanha-Gama, Roberta
Ribeiro-Pereira, Cristiane
Midlej, Victor
Benchimol, Marlene
Morandi, Verônica
Barja-Fidalgo, Christina
2016
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Helal-Neto, Edward
Brandão-Costa, Renata M.
Saldanha-Gama, Roberta
Ribeiro-Pereira, Cristiane
Midlej, Victor
Benchimol, Marlene
Morandi, Verônica
Barja-Fidalgo, Christina
Titel
Priming Endothelial Cells With a Melanoma-Derived Extracellular Matrix Triggers the Activation of αvβ3/VEGFR2 Axis
Ist Teil von
Journal of cellular physiology, 2016-11, Vol.231 (11), p.2464-2473
Ort / Verlag
United States: Blackwell Publishing Ltd
Erscheinungsjahr
2016
Quelle
MEDLINE
Beschreibungen/Notizen
The unique composition of tumor‐produced extracellular matrix (ECM) can be a determining factor in changing the profile of endothelial cells in the tumor microenvironment. As the main receptor for ECM proteins, integrins can activate a series of signaling pathways related to cell adhesion, migration, and differentiation of endothelial cells that interact with ECM proteins. We studied the direct impact of the decellularized ECM produced by a highly metastatic human melanoma cell line (MV3) on the activation of endothelial cells and identified the intracellular signaling pathways associated with cell differentiation. Our data show that compared to the ECM derived from a human melanocyte cell line (NGM‐ECM), ECM produced by a melanoma cell line (MV3‐ECM) is considerably different in ultrastructural organization and composition and possesses a higher content of tenascin‐C and laminin and a lower expression of fibronectin. When cultured directly on MV3‐ECM, endothelial cells change morphology and show increased adhesion, migration, proliferation, and tubulogenesis. Interaction of endothelial cells with MV3‐ECM induces the activation of integrin signaling, increasing FAK phosphorylation and its association with Src, which activates VEGFR2, potentiating the receptor response to VEGF. The blockage of αvβ3 integrin inhibited the FAK‐Src association and VEGFR activation, thus reducing tubulogenesis. Together, our data suggest that the interaction of endothelial cells with the melanoma‐ECM triggers integrin‐dependent signaling, leading to Src pathway activation that may potentiate VEGFR2 activation and up‐regulate angiogenesis. J. Cell. Physiol. 231: 2464–2473, 2016. © 2016 Wiley Periodicals, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0021-9541
eISSN: 1097-4652
DOI: 10.1002/jcp.25358
Titel-ID: cdi_proquest_miscellaneous_1804868098
Format
–
Schlagworte
Cell Adhesion
,
Cell Line, Tumor
,
Cell Movement
,
Cell Proliferation
,
Endothelial Cells - enzymology
,
Endothelial Cells - metabolism
,
Enzyme Activation
,
Extracellular Matrix - metabolism
,
Extracellular Matrix - ultrastructure
,
Focal Adhesion Protein-Tyrosine Kinases - metabolism
,
Humans
,
Integrin alphaVbeta3 - metabolism
,
Melanocytes - metabolism
,
Melanoma - metabolism
,
Neovascularization, Physiologic
,
Phosphorylation
,
Proto-Oncogene Proteins c-akt - metabolism
,
Signal Transduction
,
Vascular Endothelial Growth Factor Receptor-2 - metabolism
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von
bX