Plant biotechnology journal, 2016-02, Vol.14 (2), p.592-602
2016
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- Autor(en) / Beteiligte
- Titel
- myo‐inositol‐1‐phosphate synthase gene, IbMIPS1, enhances salt and drought tolerance and stem nematode resistance in transgenic sweet potato
- Ist Teil von
- Plant biotechnology journal, 2016-02, Vol.14 (2), p.592-602
- Ort / Verlag
- England: Blackwell Pub
- Erscheinungsjahr
- 2016
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Myo‐inositol‐1‐phosphate synthase (MIPS) is a key rate limiting enzyme in myo‐inositol biosynthesis. The MIPS gene has been shown to improve tolerance to abiotic stresses in several plant species. However, its role in resistance to biotic stresses has not been reported. In this study, we found that expression of the sweet potato IbMIPS1 gene was induced by NaCl, polyethylene glycol (PEG), abscisic acid (ABA) and stem nematodes. Its overexpression significantly enhanced stem nematode resistance as well as salt and drought tolerance in transgenic sweet potato under field conditions. Transcriptome and real‐time quantitative PCR analyses showed that overexpression of IbMIPS1 up‐regulated the genes involved in inositol biosynthesis, phosphatidylinositol (PI) and ABA signalling pathways, stress responses, photosynthesis and ROS‐scavenging system under salt, drought and stem nematode stresses. Inositol, inositol‐1,4,5‐trisphosphate (IP₃), phosphatidic acid (PA), Ca²⁺, ABA, K⁺, proline and trehalose content was significantly increased, whereas malonaldehyde (MDA), Na⁺ and H₂O₂ content was significantly decreased in the transgenic plants under salt and drought stresses. After stem nematode infection, the significant increase of inositol, IP₃, PA, Ca²⁺, ABA, callose and lignin content and significant reduction of MDA content were found, and a rapid increase of H₂O₂ levels was observed, peaked at 1 to 2 days and thereafter declined in the transgenic plants. This study indicates that the IbMIPS1 gene has the potential to be used to improve the resistance to biotic and abiotic stresses in plants.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1467-7644eISSN: 1467-7652DOI: 10.1111/pbi.12402Titel-ID: cdi_proquest_miscellaneous_1800487224
- Format
- –
- Schlagworte
- Abiotic stress, Abscisic acid, Abscisic Acid - pharmacology, Adaptation, Physiological - drug effects, Adaptation, Physiological - genetics, Agricultural production, Animals, Biosynthesis, Biotechnology, biotic stress, Calcium, Calcium ions, callose, Carbenicillin, Crop diseases, Crops, Disease Resistance - drug effects, Drought, Drought resistance, drought tolerance, Droughts, Food, Gene expression, gene expression regulation, gene overexpression, Genes, Genes, Plant, Genetic engineering, Genetic research, Genetically altered foods, Genetically engineered foods, Genetically modified plants, Hydrogen peroxide, IbMIPS1, Infections, Inositol, Inositol 1,4,5-trisphosphate receptors, inositol-3-phosphate synthase, Inositols, Ipomoea batatas - enzymology, Ipomoea batatas - genetics, Ipomoea batatas - parasitology, Ipomoea batatas - physiology, Life sciences, Lignin, Malondialdehyde, MIPS gene, myo-inositol, Myo-Inositol-1-Phosphate Synthase - genetics, Myo-Inositol-1-Phosphate Synthase - metabolism, Nematoda, Nematoda - drug effects, Nematoda - physiology, nematode infections, Nematodes, Phosphates, Phosphatidic acid, Phosphatidylinositol, Photosynthesis, Plant Diseases - parasitology, Plant species, Plant Stems - drug effects, Plant Stems - parasitology, Plants (botany), Plants (organisms), Plants, Genetically Modified, Polyethylene glycol, Polyethylene Glycols - pharmacology, potassium, Potatoes, Proline, quantitative polymerase chain reaction, Rice, salt and drought tolerance, Salt-Tolerance - drug effects, Salt-Tolerance - genetics, Salts, Scavenging, Signal transduction, Signal Transduction - drug effects, sodium, Sodium chloride, Sodium Chloride - pharmacology, Solanum tuberosum, Soybeans, stem nematode resistance, stress response, Stress, Physiological - drug effects, Stresses, sweet potato, Sweet potatoes, transcriptome, Transgenic, Transgenic plants, Trehalose, Up-Regulation - drug effects, Up-Regulation - genetics