Details

Autor(en) / Beteiligte

• Alamein, Mohammad A.
• Liu, Qin
• Stephens, Sebastien
• Skabo, Stuart
• Warnke, Frauke
• Bourke, Robert
• Heiner, Peter
• Warnke, Patrick H.

Titel

Nanospiderwebs: Artificial 3D Extracellular Matrix from Nanofibers by Novel Clinical Grade Electrospinning for Stem Cell Delivery

Ist Teil von

• Advanced healthcare materials, 2013-05, Vol.2 (5), p.702-717

Ort / Verlag

Weinheim: WILEY-VCH Verlag

Erscheinungsjahr

2013

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Novel clinical grade electrospinning methods could provide three‐dimensional (3D) nanostructured biomaterials comprising of synthetic or natural biopolymer nanofibers. Such advanced materials could potentially mimic the natural extracellular matrix (ECM) accurately and may provide superior niche‐like spaces on the subcellular scale for optimal stem‐cell attachment and individual cell homing in regenerative therapies. The goal of this study was to design several novel “nanofibrous extracellular matrices” (NF‐ECMs) with a natural mesh‐like 3D architecture through a unique needle‐free multi‐jet electrospinning method in highly controlled manner to comply with good manufacturing practices (GMP) for the production of advanced healthcare materials for regenerative medicine, and to test cellular behavior of human mesenchymal stem cells (HMSCs) on these. Biopolymers manufactured as 3D NF‐ECM meshes under clinical grade GMP‐like conditions show higher intrinsic cytobiocompatibility with superior cell integration and proliferation if compared to their 2D counterparts or a clinically‐approved collagen membrane. A novel electrospinning process for engineering three‐dimensional nanofi‐brous extracellular matrixes (NFECMs) is initiated using a NanoSpider machine. Under clinical grade goodmanufacturing‐practice‐like conditions, several NF‐ECMs are designed in a controlled manner to yield highly uniform fibers with sub‐micrometer dimensions, favourable biomechanical properties and intrinsic cytobiocompatility. This permits both the proliferation and differentiation of human mesenchymal stem cells when provided appropriate biological cues.