UNIVERSI
TÄ
TS-
BIBLIOTHEK
P
ADERBORN
Anmelden
Menü
Menü
Start
Hilfe
Blog
Weitere Dienste
Neuerwerbungslisten
Fachsystematik Bücher
Erwerbungsvorschlag
Bestellung aus dem Magazin
Fernleihe
Einstellungen
Sprache
Deutsch
Deutsch
Englisch
Farbschema
Hell
Dunkel
Automatisch
Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist
gegebenenfalls
nur via VPN oder Shibboleth (DFN-AAI) möglich.
mehr Informationen...
Universitätsbibliothek
Katalog
Suche
Details
Zur Ergebnisliste
Ergebnis 21 von 24
Datensatz exportieren als...
BibTeX
Identification of transcriptome profiles for the DNA-damaging agents bleomycin and hydrogen peroxide in L5178Y mouse lymphoma cells
Environmental and molecular mutagenesis, 2003, Vol.42 (1), p.19-25
Seidel, Shawn D.
Kan, H. Lynn
Stott, William T.
Schisler, Melissa R.
Gollapudi, B. Bhaskar
2003
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Seidel, Shawn D.
Kan, H. Lynn
Stott, William T.
Schisler, Melissa R.
Gollapudi, B. Bhaskar
Titel
Identification of transcriptome profiles for the DNA-damaging agents bleomycin and hydrogen peroxide in L5178Y mouse lymphoma cells
Ist Teil von
Environmental and molecular mutagenesis, 2003, Vol.42 (1), p.19-25
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2003
Quelle
Wiley Blackwell Single Titles
Beschreibungen/Notizen
It is believed that some aspects of genotoxicity are associated with changes in the transcription levels of certain genes, especially those involved in DNA repair and cell cycle control. Additionally, it is hypothesized that chemicals sharing a common mode of genotoxicity should exhibit similar changes in gene expression. We have evaluated these hypotheses by analyzing transcriptome profiles of mouse lymphoma L5178Y/TK+/− cells treated with bleomycin and hydrogen peroxide, two mutagens that produce genotoxicity by generating reactive free radicals. The cells were treated for 4 hr and RNA was isolated at the end of the treatment and after a 20 hr recovery. Transcriptome analyses were performed using the Clontech Mouse 1.2K cDNA microarray (1,185 genes) and hybridization with a 32[P]‐labeled probe. Of the genes examined, each mutagen altered the expression (1.5‐fold or greater) of only two genes after the 4 hr treatment. In cells allowed to recover for 20 hr after treatment, bleomycin and hydrogen peroxide altered the expression of 8 and 5 genes, respectively. Many of the altered genes have some association with apoptosis. Of these genes, three (the genes encoding granzyme A, integrin beta 7, and 45 kDa calcium‐binding protein precursor) were in common between chemical treatments. The expression of DNA repair and cell cycle controlling genes present on the array was not affected by the treatments. These results show that bleomycin and hydrogen peroxide both have unique and commonly regulated genes that have the potential to serve as biomarkers of exposure to agents causing DNA damage by free radical mechanisms. Environ. Mol. Mutagen. 42:19–25, 2003. © 2003 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0893-6692
eISSN: 1098-2280
DOI: 10.1002/em.10169
Titel-ID: cdi_proquest_miscellaneous_17987285
Format
–
Schlagworte
Animals
,
Antimetabolites, Antineoplastic - toxicity
,
Biological and medical sciences
,
bleomycin
,
Bleomycin - toxicity
,
DNA Damage
,
Dose-Response Relationship, Drug
,
Fundamental and applied biological sciences. Psychology
,
Gene Expression Profiling
,
Gene Expression Regulation, Neoplastic - drug effects
,
Hematologic and hematopoietic diseases
,
hydrogen peroxide
,
Hydrogen Peroxide - toxicity
,
Leukemia L5178 - genetics
,
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
,
Medical sciences
,
Mice
,
Molecular and cellular biology
,
Molecular genetics
,
mouse lymphoma L5178Y cells
,
Mutagenesis. Repair
,
Oligonucleotide Array Sequence Analysis
,
Pharmacogenetics - methods
,
RNA, Messenger - metabolism
,
RNA, Neoplasm - analysis
,
toxicogenomics
,
Transcription, Genetic - drug effects
,
Transcription, Genetic - genetics
,
Tumor Cells, Cultured
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von
bX