Details

Autor(en) / Beteiligte

• Reis, F.B. dos Jr
• Silva, M.F. da
• Teixeira, K.R.S
• Urquiaga, S
• Reis, V.M

Titel

Intra-specific diversity study of the nitrogen fixing bacterium Azospirillum amazonense isolated from different Brachiaria species

Ist Teil von

• Symbiosis (Philadelphia, Pa.), 2004, Vol.36 (1), p.41-56

Ort / Verlag

Philadelphia, PA: Balaban

Erscheinungsjahr

2004

Beschreibungen/Notizen

• The limitation of nitrogen is considered one of the most important factors for the Brazilian pastures degradation. However, there are evidences that pastures formed by some species of the Brachiaria genus could be benefited by the biological nitrogen fixation (BNF), guaranteeing a higher longevity to these pastures. Previous studies showed that the diazotrophic bacteria found in association with these forage grasses were mainly from the Azospirillum amazonense species. Since associations between these microorganisms and plants are generally conditioned by the vegetation, it is possible that different Brachiaria genotypes can exercise a selective effect on the Azospirillum populations. The aim of this work was to study the intra-specific diversity of A. amazonense isolates and to establish possible influences of different Brachiaria species and edaphoclimatic conditions. The characterisation of the diversity among these isolates was conducted using serological tests (ELISA - Enzyme Linked Immunosorbent Assay) and tests of carbon sources metabolisation (BIOLOG super(TM)). These methods were capable to show important diversity among the isolates of A. amazonense.