Biochemistry (Moscow), 2016-04, Vol.81 (4), p.420-427
2016
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Details
- Autor(en) / Beteiligte
- Titel
- Construction of a fusion enzyme exhibiting superoxide dismutase and peroxidase activity
- Ist Teil von
- Biochemistry (Moscow), 2016-04, Vol.81 (4), p.420-427
- Ort / Verlag
- Moscow: Pleiades Publishing
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- A chimeric gene construct encoding human peroxiredoxin 6 and Mn-superoxide dismutase from Escherichia coli was developed. Conditions for expression of the fusion protein in E. coli cell were optimized. Fusing of the enzymes into a single polypeptide chain with peroxiredoxin 6 at the N -terminus (PSH) did not affect their activities. On the contrary, the chimeric protein with reverse order of enzymes (SPH) was not obtained in a water-soluble active form. The active chimeric protein (PSH) exhibiting both peroxidase and superoxide dismutase activities was prepared and its physicochemical properties were characterized.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-2979eISSN: 1608-3040DOI: 10.1134/S0006297916040131Titel-ID: cdi_proquest_miscellaneous_1796685341
- Format
- –
- Schlagworte
- Biochemistry, Biomedical and Life Sciences, Biomedicine, Bioorganic Chemistry, E coli, Enzymes, Escherichia coli, Escherichia coli - metabolism, Escherichia coli Proteins - genetics, Escherichia coli Proteins - metabolism, Gene expression, Genetic aspects, Humans, Life Sciences, Microbiology, Observations, Oxidative stress, Peroxidase, Peroxiredoxin VI - genetics, Peroxiredoxin VI - metabolism, Physicochemical properties, Physiological aspects, Protein Stability, Protein-protein interactions, Recombinant Fusion Proteins - biosynthesis, Recombinant Fusion Proteins - chemistry, Recombinant Fusion Proteins - isolation & purification, Superoxide, Superoxide Dismutase - genetics, Superoxide Dismutase - metabolism, Temperature