Details

Autor(en) / Beteiligte

• Moretti, Eduardo Gregolin
• Yujra, Veronica Quispe
• Claudio, Samuel Rangel
• Silva, Marcelo Jose Dias
• Vilegas, Wagner
• Pereira, Camilo Dias Seabra
• de Oliveira, Flavia
• Ribeiro, Daniel Araki

Titel

Acute crack cocaine exposure induces genetic damage in multiple organs of rats

Ist Teil von

• Environmental science and pollution research international, 2016-04, Vol.23 (8), p.8104-8112

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Crack cocaine is a very toxic product derived from cocaine. The aim of this study was to evaluate genetic damage in multiple organs of rats following acute exposure to crack cocaine. A total of 20 Wistar rats were distributed into four groups ( n  = 5), as follows: 0, 4.5, 9, and 18 mg/kg body weight (b.w.) of crack cocaine administered by intraperitoneal route (i.p.). All animals were killed 24 h after intraperitoneal (i.p.) injection. The results showed that crack cocaine increased the number of micronucleated cells in bone marrow cells exposed to 18 mg/kg crack cocaine ( p  < 0.05). Peripheral blood and liver cells presented genetic damage as depicted by single cell gel (comet) assay at 9 and 18 mg/kg doses ( p  < 0.05). Immunohistochemistry data revealed significant increase in 8-hydroxy-20-deoxyguanosine (8-OHdG) immunoexpression in hepatocytes of animals exposed to crack cocaine at 9 and 18 mg/kg ( p  < 0.05) when compared with negative controls. Taken together, our results demonstrate that crack cocaine is able to induce genomic damage in multiple organs of Wistar rats.