Details

Autor(en) / Beteiligte

• Pan, Fengshan
• Meng, Qian
• Wang, Qiong
• Luo, Sha
• Chen, Bao
• Khan, Kiran Yasmin
• Yang, Xiaoe
• Feng, Ying

Titel

Endophytic bacterium Sphingomonas SaMR12 promotes cadmium accumulation by increasing glutathione biosynthesis in Sedum alfredii Hance

Ist Teil von

• Chemosphere (Oxford), 2016-07, Vol.154, p.358-366

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• A hydroponic experiment was conducted to verify the effects of inoculation with endophytic bacteria Sphingomonas SaMR12 on root growth, cadmium (Cd) uptake, reactive oxygen species (ROS), antioxidases, glutathione (GSH) and the related gene expression of Sedum alfredii Hance under different levels of Cd such as 0, 10, 25, 100 and 400 μM. The results showed that inoculation of SaMR12 improved Cd accumulation and upregulated glutathione synthase (GS) expression, but slightly reduced malondialdehyde (MDA) concentration and alleviated Cd-induced damage in roots. However it didn't alter the activities of antioxidant enzymes. When Cd concentration exceeded 25 μM, SaMR12 increased the concentration of GSH and the expression level of GSH1. At high Cd treatment levels (100 and 400 μM), SaMR12 significantly reduced H2O2 concentration and enhanced expression level of 1-Cys peroxiredoxin PER1 and ATPS genes. These results indicate that although SaMR12 has no significant effects on antioxidases activities, it reduces H2O2 concentration by enhancing GSH concentration and relevant genes expression, and subsequently improves Cd tolerance and accumulation. •The antioxidant enzymes of S. alfredii root were not sensitive to Cd stress and SaMR12.•SaMR12 significantly reduced root H2O2 concentration under high Cd stress.•SaMR12 increased root GSH concentration and its biosynthetic genes expression.•SaMR12 improved Cd accumulation by affecting root GSH and ROS metabolisms.