Details

Autor(en) / Beteiligte

• Okada, Sachiko
• Sone, Takefumi
• Fujisawa, Masaki
• Nakayama, Shigeki
• Takenaka, Mizuki
• Ishizaki, Kimitsune
• Kono, Kaoru
• Shimizu-Ueda, Yuu
• Hanajiri, Tsutomu
• Yamato, Katsuyuki T.
• Fukuzawa, Hideya
• Brennicke, Axel
• Ohyama, Kanji

Titel

The Y Chromosome in the Liverwort Marchantia polymorpha has Accumulated Unique Repeat Sequences Harboring a Male-Specific Gene

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2001-07, Vol.98 (16), p.9454-9459

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2001

Quelle

MEDLINE

Beschreibungen/Notizen

• The haploid liverwort Marchantia polymorpha has heteromorphic sex chromosomes, an X chromosome in the female and a Y chromosome in the male. We here report on the repetitive structure of the liverwort Y chromosome through the analysis of male-specific P1-derived artificial chromosome (PAC) clones, pMM4G7 and pMM23-130F12. Several chromosome-specific sequence elements of ≈70 to 400 nt are combined into larger arrangements, which in turn are assembled into extensive Y chromosome-specific stretches. These repeat sequences contribute 2-3 Mb to the Y chromosome based on the observations of three different approaches: fluorescence in situ hybridization, dot blot hybridization, and the frequency of clones containing the repeat sequences in the genomic library. A novel Y chromosome-specific gene family was found embedded among these repeat sequences. This gene family encodes a putative protein with a RING finger motif and is expressed specifically in male sexual organs. To our knowledge, there have been no other reports for an active Y chromosome-specific gene in plants. The chromosome-specific repeat sequences possibly contribute to determining the identity of the Y chromosome in M. polymorpha as well as to maintaining genes required for male functions, as in mammals such as human.