Details

Autor(en) / Beteiligte

• Dai, Lianpan
• Song, Jian
• Lu, Xishan
• Deng, Yong-Qiang
• Musyoki, Abednego Moki
• Cheng, Huijun
• Zhang, Yanfang
• Yuan, Yuan
• Song, Hao
• Haywood, Joel
• Xiao, Haixia
• Yan, Jinghua
• Shi, Yi
• Qin, Cheng-Feng
• Qi, Jianxun
• Gao, George F.

Titel

Structures of the Zika Virus Envelope Protein and Its Complex with a Flavivirus Broadly Protective Antibody

Ist Teil von

• Cell host & microbe, 2016-05, Vol.19 (5), p.696-704

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2016

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Zika virus (ZIKV), a mosquito-borne flavivirus, is a current global public health concern. The flavivirus envelope (E) glycoprotein is responsible for virus entry and represents a major target of neutralizing antibodies for other flaviviruses. Here, we report the structures of ZIKV E protein at 2.0 Å and in complex with a flavivirus broadly neutralizing murine antibody 2A10G6 at 3.0 Å. ZIKV-E resembles all the known flavivirus E structures but contains a unique, positively charged patch adjacent to the fusion loop region of the juxtaposed monomer, which may influence host attachment. The ZIKV-E-2A10G6 complex structure reveals antibody recognition of a highly conserved fusion loop. 2A10G6 binds to ZIKV-E with high affinity in vitro and neutralizes currently circulating ZIKV strains in vitro and in mice. The E protein fusion loop epitope represents a potential candidate for therapeutic antibodies against ZIKV. [Display omitted] •Report structures of ZIKV E protein and its complex with a broadly neutralizing antibody•ZIKV-E contains a unique, positively charged patch, which may influence host attachment•The ZIKV-E-antibody structure reveals antibody recognition of a conserved fusion loop•Antibody binds ZIKV-E and neutralizes circulating ZIKV strains in vitro and in mice Zika virus (ZIKV) is a mosquito-borne flavivirus. Dai et al. report the structures of ZIKV envelope (E) protein and its complex with a flavivirus broadly protective antibody, which reveals antibody recognition of a conserved fusion loop. Antibody binds to ZIKV-E with high affinity and neutralizes currently circulating ZIKV strains in mice.