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Field evaluation of lentil cultivars inoculated with Rhizobium leguminosarum bv. viciae strains for nitrogen fixation using nitrogen-15 isotope dilution
Ist Teil von
Biology and fertility of soils, 2000, Vol.31 (1), p.65-69
A super(15)N isotope dilution technique was applied to quantify the extent of N sub(2) fixation in lentil (Lens culinaris Medik.) cultivars as influenced by Rhizobium leguminosarum bv. viciae strains in a field experiment in Pakistan. The experiment was conducted on a soil with a very small indigenous rhizobial population and where N was a limiting factor for crop production. Significant variations in number of nodules, dry weight of nodules, biomass yield, grain yield, total N yield, proportion of plant N derived from N sub(2) fixation (P sub(fix)) and amount of N derived from the atmosphere (N sub(dfa)) were observed among combined treatments of four rhizobial strains and six lentil varieties. In a field previously labelled with super(15)N, to which a basal dose of 75 kg P sub(2)O sub(5) ha super(-1) was applied as single super phosphate, N sub(dfa) ranged from 15 to 24 kg N ha super(-1) when calculated according to rhizobial strain and from 4 to 38 kg N ha super(-1) when calculated according to lentil variety. Lc 26 was the most effective strain and fixed 243% more N than the indigenous population in the uninoculated control. In treatments with the lentil variety PL-406, N sub(dfa) was 38 kg N ha super(-1), which was 850% higher than with the lentil variety Precoz/F6-20-1xM-85. Generally, the varieties with greater P sub(fix) produced a higher dry matter yield.