Details

Autor(en) / Beteiligte

• Prensner, John R
• Chen, Wei
• Han, Sumin
• Iyer, Matthew K
• Cao, Qi
• Kothari, Vishal
• Evans, Joseph R
• Knudsen, Karen E
• Paulsen, Michelle T
• Ljungman, Mats
• Lawrence, Theodore S
• Chinnaiyan, Arul M
• Feng, Felix Y

Titel

The Long Non-Coding RNA PCAT-1 Promotes Prostate Cancer Cell Proliferation through cMyc

Ist Teil von

• Neoplasia (New York, N.Y.), 2014-11, Vol.16 (11), p.900-908

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• Abstract Long non-coding RNAs (lncRNAs) represent an emerging layer of cancer biology, contributing to tumor proliferation, invasion, and metastasis. Here, we describe a role for the oncogenic lncRNA PCAT-1 in prostate cancer proliferation through cMyc. We find that PCAT-1 –mediated proliferation is dependent on cMyc protein stabilization, and using expression profiling, we observed that cMyc is required for a subset of PCAT-1 –induced expression changes. The PCAT-1 –cMyc relationship is mediated through the post-transcriptional activity of the MYC 3′ untranslated region, and we characterize a role for PCAT-1 in the disruption of MYC -targeting microRNAs. To further elucidate a role for post-transcriptional regulation, we demonstrate that targeting PCAT-1 with miR-3667-3p, which does not target MYC , is able to reverse the stabilization of cMyc by PCAT-1 . This work establishes a basis for the oncogenic role of PCAT-1 in cancer cell proliferation and is the first study to implicate lncRNAs in the regulation of cMyc in prostate cancer.