Toxicon (Oxford), 2016-02, Vol.110, p.74-78
2016
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- Autor(en) / Beteiligte
- Titel
- The nematocysts venom of Chrysaora helvola Brandt leads to apoptosis-like cell death accompanied by uncoupling of oxidative phosphorylation
- Ist Teil von
- Toxicon (Oxford), 2016-02, Vol.110, p.74-78
- Ort / Verlag
- England: Elsevier Ltd
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The present work investigated the effects of the nematocysts venom (NV) from the Chrysaora helvola Brandt (C. helvola) jellyfish on the human nasopharyngeal carcinoma cell line, CNE-2. The medium lethal concentration (LC50), quantified by MTT assays, was 1.7 ± 0.53 μg/mL (n = 5). An atypical apoptosis-like cell death was confirmed by LDH release assay and Annexin V-FITC/PI staining-based flow cytometry. Interestingly, activation of caspase-4 other than caspase-3, -8, -9 and -1 was observed. Moreover, the NV stimuli caused a time-dependent loss of mitochondrial membrane potential (ΔΨm) as was an intracellular ROS burst. These results indicated that there was uncoupling of oxidative phosphorylation (UOP). An examination of the intracellular pH value by a pH-sensitive fluorescent probe, BCECF, suggested that the UOP was due to the time-dependent increase in the intracellular pH. This is the first report that jellyfish venom can induce UOP. •Nematocyst venom from Chrysaora helvola Brandt induces apoptosis-like cell death on CNE-2 cells.•Nematocyst venom induces ΔΨm collapse and uncoupling of oxidative phosphorylation.•Nematocyst venom induces an increase of intracellular pH.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0041-0101eISSN: 1879-3150DOI: 10.1016/j.toxicon.2015.12.011Titel-ID: cdi_proquest_miscellaneous_1786202546
- Format
- –
- Schlagworte
- Animals, Antineoplastic Agents - isolation & purification, Antineoplastic Agents - pharmacology, Apoptosis - drug effects, Assaying, Atypical apoptosis, Biotechnology, Carcinoma - drug therapy, Carcinoma - metabolism, Caspase-4, Cell death, Cell Line, Tumor, Cell Survival - drug effects, China, Chrysaora, Chrysaora helvola Brandt, CNE-2, Cnidarian Venoms - isolation & purification, Cnidarian Venoms - pharmacology, Drug Discovery, Flow cytometry, Human, Humans, Hydrogen-Ion Concentration, Kinetics, Lethal Dose 50, Membrane Potential, Mitochondrial - drug effects, Mitochondrial transmembrane potential, Nasopharyngeal Neoplasms - drug therapy, Nasopharyngeal Neoplasms - metabolism, Nematocyst - chemistry, Nematocyst - growth & development, Nematocysts venom, Oxidative Phosphorylation - drug effects, Pacific Ocean, Phosphorylation, Reactive Oxygen Species - agonists, Reactive Oxygen Species - metabolism, Respiratory Mucosa - drug effects, Scyphozoa - chemistry, Scyphozoa - growth & development, Stimuli, Uncoupling Agents - isolation & purification, Uncoupling Agents - pharmacology, Uncoupling of oxidative phosphorylation