Details

Autor(en) / Beteiligte

• Shkryl, Yury N
• Bulgakov, Victor P
• Veremeichik, Galina N
• Kovalchuk, Svetlana N
• Kozhemyako, Valery B
• Kamenev, Dmitrii G
• Semiletova, Irina V
• Timofeeva, Yana O
• Shchipunov, Yury A
• Kulchin, Yury N

Titel

Bioinspired enzymatic synthesis of silica nanocrystals provided by recombinant silicatein from the marine sponge Latrunculia oparinae

Ist Teil von

• Bioprocess and biosystems engineering, 2016-01, Vol.39 (1), p.53-58

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• The process of silica formation in marine sponges is thought to be mediated by a family of catalytically active structure-directing enzymes called silicateins. It has been demonstrated in biomimicking syntheses that silicateins facilitated the formation of amorphous SiO₂. Here, we present evidence that the silicatein LoSiLA1 from the marine sponge Latrunculia oparinae catalyzes the in vitro synthesis of hexa-tetrahedral SiO₂ crystals of 200–300 nm. This was possible in the presence of the silica precursor tetrakis-(2-hydroxyethyl)-orthosilicate that is completely soluble in water and biocompatible, experiences hydrolysis–condensation at neutral pH and ambient conditions.