Details

Autor(en) / Beteiligte

• Mohammadpour, Hemn
• Pourfathollah, Ali Akbar
• Zarif, Mahin Nikougoftar
• Hashemi, Sayed Mahmoud

Titel

Increasing proliferation of murine adipose tissue-derived mesenchymal stem cells by TNF-[alpha] plus IFN-[gamma]

Ist Teil von

• Immunopharmacology and immunotoxicology, 2016-01, Vol.38 (2), p.68-76

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Taylor & Francis Journals Auto-Holdings Collection

Beschreibungen/Notizen

• The objective of the current study was to assess the effects of tumor necrosis factor-[alpha] (TNF-[alpha]) and interferon-[gamma] (IFN-[gamma]) along with their simultaneous application on proliferation and pluripotency genes of murine adipose tissue-derived mesenchymal stem cells (AT-MSCs). The proliferation, doubling time (DT), colony-forming unit-fibroblast (CFU-F), pluripotency genes expression, and proliferation-related immunomodulatory markers of MSCs were analyzed upon activation with TNF-[alpha] (10ng/ml), IFN-[gamma] (10ng/ml) and both TNF-[alpha] and IFN-[gamma] (5ng/ml+5ng/ml). Pluripotency genes including Oct-4, Sox-2, and Nanog as well as proliferation-associated immunomodulatory cytokines such as insulin-like growth factor 1 (IGF-1) and transforming growth factor-[beta] (TGF-[beta]) expression were evaluated using real-time PCR. Surface expression of Qa2 (HLA-G) was analyzed by flow cytometry. Pretreatment of MSCs with TNF-[alpha] plus IFN-[gamma] led to significantly increased proliferation, DT and CFU-F as well as expression of pluripotency genes in AT-MSCs (p < 0.01). MSCs expressed more IGF-1, TGF-[beta], and Qa2 upon activation with TNF-[alpha] plus IFN-[gamma] and IFN-[gamma]. MSCs expressed significantly decreased amounts of TGF-[beta] and Qa2 in presence of TNF-[alpha]. TNF-[alpha] combined with IFN-[gamma] may be improved the proliferation of AT-MSCs. Conversely, expanded MSCs pointed out low levels of the immunomodulatory marker, s especially Qa2 in the presence of TNF-[alpha]. In conclusion, we showed that TNF-[alpha] together with IFN-[gamma] increased the proliferation of MSCs and slightly enhanced the expression of pluripotency genes.