Details

Autor(en) / Beteiligte

• Zhao, Cheng
• Xie, Shangxian
• Pu, Yunqiao
• Zhang, Rui
• Huang, Fang
• Ragauskas, Arthur J
• Yuan, Joshua S

Titel

Synergistic enzymatic and microbial lignin conversion

Ist Teil von

• Green chemistry : an international journal and green chemistry resource : GC, 2016-01, Vol.18 (5), p.1306-1312

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The utilization of lignin for fungible fuels and chemicals represents one of the most imminent challenges in modern biorefineries. However, bioconversion of lignin is highly challenging due to its recalcitrant nature as a phenolic heteropolymer. This study addressed the challenges by revealing the chemical and biological mechanisms for synergistic lignin degradation by a bacterial and enzymatic system, which significantly improved lignin consumption, cell growth and lipid yield. The Rhodococcus opacuscell growth increased exponentially in response to the level of laccase treatment, indicating the synergy between laccase and bacterial cells in lignin degradation. Other treatments like iron and hydrogen peroxide showed limited impact on cell growth. Chemical analysis of lignin under various treatments further confirmed the synergy between laccase and cells at the chemical level. super(31)P nuclear magnetic resonance (NMR) suggested that laccase, R. opacuscell and Fenton reaction reagents promoted the degradation of different types of lignin functional groups, elucidating the chemical basis for the synergistic effects. super(31)P NMR further revealed that laccase treatment had the most significant impact for degrading the abundant chemical groups. The results were further confirmed by the molecular weight analysis and lignin quantification by the Prussian blue assay. The cell-laccase fermentation led to a 17-fold increase of lipid production. Overall, the study indicated that laccase and R. opacuscan synergize to degrade lignin efficiently, likely through rapid utilization of monomers generated by laccase to promote the reaction toward depolymerization. The study provided a potential path for more efficient lignin conversion and development of consolidated lignin conversion.