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Contents
Specific scope
410
1. Introduction
411
2. Identity
411
3. Detection
411
4. Identification
412
4.1. Morphological identification
412
4.1.1. Eggs
412
4.1.2. Larvae
412
4.1.2.1. Introduction
412
4.1.2.2. General description Spodoptera larvae
413
4.1.2.3. Spodoptera littoralis and S. litura
414
4.1.2.4. Spodoptera eridania
417
4.1.2.5. Spodoptera frugiperda
419
4.1.2.6. Possible confusion with Spodoptera exigua
421
4.1.3. Pupae
422
4.1.3.1. General description
422
4.1.3.2. Possible confusion with Spodoptera exigua
422
4.1.4. Adults
422
4.1.4.1. Introduction
422
4.1.4.2. Spodoptera littoralis
424
4.1.4.3. Spodoptera litura
425
4.1.4.4. Spodoptera eridania
427
4.1.4.5. Spodoptera frugiperda
429
4.2. Molecular methods
430
5. Reference material
430
6. Reporting and documentation
430
7. Performance criteria
430
8. Further information
431
9. Feedback on this diagnostic protocol
431
10. Protocol revision
431
11. Acknowledgements
431
12. References
431
Appendix 1: Features of Lepidoptera larvae
433
Appendix 2: Keys to adults based on external morphology
434
Appendix 3: Information on genitalia
435
Appendix 4: Identification of Spodoptera eridania and S. frugiperda using real‐time PCR
441
Appendix 5: Identification of Spodoptera littoralis and S. litura using real‐time PCR
442
Specific scope
This Standard provides guidance for the identification of S. littoralis, S. litura, S. frugiperda and S. eridania.
Specific approval and amendment
This Standard was approved in 2015‐09.