Details

Autor(en) / Beteiligte

• Liu, Wei-Ren
• Jin, Lei
• Tian, Meng-Xin
• Jiang, Xi-Fei
• Yang, Liu-Xiao
• Ding, Zhen-Bin
• Shen, Ying-Hao
• Peng, Yuan-Fei
• Gao, Dong-Mei
• Zhou, Jian
• Qiu, Shuang-Jian
• Dai, Zhi
• Fan, Jia
• Shi, Ying-Hong

Titel

Caveolin-1 promotes tumor growth and metastasis via autophagy inhibition in hepatocellular carcinoma

Ist Teil von

• Clinics and research in hepatology and gastroenterology, 2016-04, Vol.40 (2), p.169-178

Ort / Verlag

France

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Summary Background Caveolin-1 is a member of the caveolae family of membrane proteins. Although some researchers have investigated the function of Caveolin-1 in hepatocellular carcinoma, the mechanism of Caveolin-1 action and its prognostic value in hepatocellular carcinoma remain unclear. Methods Caveolin-1 expression was measured in hepatocellular carcinoma cell lines and tissues using quantitative reverse transcription-polymerase chain reaction, western blot, and immunofluorescence assays. In in vitro experiments, Caveolin-1 was depleted using a short hairpin RNA lentiviral vector, and tumor cell behavior was analyzed. The effect of Caveolin-1 on hepatocellular carcinoma cell autophagy was investigated. Prognostic value of Caveolin-1 was analyzed by immunohistochemistry in two cohorts that included a total of 721 hepatocellular carcinoma patients. Results We found that Caveolin-1 was overexpressed in highly metastatic hepatocellular carcinoma cell lines and tumor tissues. Moreover, Caveolin-1 promoted hepatocellular carcinoma cell proliferation, migration, and angiogenesis and inhibited autophagy. Finally, Caveolin-1 expression in hepatocellular carcinoma tissues was inversely correlated with patient overall survival and time to recurrence. Conclusion Our data obtained from cell lines suggest an oncogenic role for Caveolin-1 in hepatocellular carcinoma, Caveolin-1 contributed to hepatocellular carcinoma cell autophagy deficiency. Furthermore, Caveolin-1 may function as a novel prognostic indicator for hepatocellular carcinoma patients after curative resection, and combination of targeted therapy aimed at Caveolin-1 and autophagy modulation may represent an effective way to treat hepatocellular carcinoma.