Details

Autor(en) / Beteiligte

• Kocic, Danijela
• Andrew Shalliker, R

Titel

new approach to live reaction monitoring using active flow technology in ultra‐high‐speed HPLC with mass spectral detection

Ist Teil von

• Journal of mass spectrometry., 2015-12, Vol.50 (12), p.1396-1399

Ort / Verlag

England: Wiley

Erscheinungsjahr

2015

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• A new type of chromatography column referred to as a parallel segmented flow (PSF) column enables ultra‐high‐speed high‐performance liquid chromatography‐MS to be undertaken. This occurs because the separation efficiency obtained on PSF columns has been shown in prior studies to be superior to conventional columns, and the flow stream is split radially inside the outlet end fitting of the column, rather than in an axial post‐column flow stream split. As a result, the flow through the column can be five times higher than the flow through the MS. In this work, the degradation of amino acids in dilute nitric acid was used to illustrate the process. Separations were obtained in less than 12 s, although the reinjection process was initiated 6 s after the previous injection. The degradation rate constant of tryptophan, in the presence of tyrosine and phenylalanine, was determined. Copyright © 2015 John Wiley & Sons, Ltd.