Details

Autor(en) / Beteiligte

• Valta, Maija P.
• Zhao, Hongjuan
• Saar, Matthias
• Tuomela, Johanna
• Nolley, Rosalie
• Linxweiler, Johannes
• Sandholm, Jouko
• Lehtimäki, Jaakko
• Härkönen, Pirkko
• Coleman, Ilsa
• Nelson, Peter S.
• Corey, Eva
• Peehl, Donna M.

Titel

Spheroid culture of LuCaP 136 patient-derived xenograft enables versatile preclinical models of prostate cancer

Ist Teil von

• Clinical & experimental metastasis, 2016-04, Vol.33 (4), p.325-337

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2016

Link zum Volltext

Quelle

SpringerNature Journals

Beschreibungen/Notizen

• LuCaP serially transplantable patient-derived xenografts (PDXs) are valuable preclinical models of locally advanced or metastatic prostate cancer. Using spheroid culture methodology, we recently established cell lines from several LuCaP PDXs. Here, we characterized in depth the features of xenografts derived from LuCaP 136 spheroid cultures and found faithful retention of the phenotype of the original PDX. In vitro culture enabled luciferase transfection into LuCaP 136 spheroids, facilitating in vivo imaging. We showed that LuCaP 136 spheroids formed intratibial, orthotopic, and subcutaneous tumors when re-introduced into mice. Intratibial tumors responded to castration and were highly osteosclerotic. LuCaP 136 is a realistic in vitro–in vivo preclinical model of a subtype of bone metastatic prostate cancer.