Carcinogenesis (New York), 2000-11, Vol.21 (11), p.2005-2010
2000
Details
- Autor(en) / Beteiligte
- Titel
- Differentially expressed genes in asbestos-induced tumorigenic human bronchial epithelial cells: implication for mechanism
- Ist Teil von
- Carcinogenesis (New York), 2000-11, Vol.21 (11), p.2005-2010
- Ort / Verlag
- Oxford: Oxford University Press
- Erscheinungsjahr
- 2000
- Link zum Volltext
- Quelle
- Oxford Journals 2020 Medicine
- Beschreibungen/Notizen
- Although exposure to asbestos fibers is associated with the development of lung cancer, the underlying mechanism(s) remains unclear. Using human papillomavirus-immortalized human bronchial epithelial (BEP2D) cells, we previously showed that UICC chrysotiles can malignantly transform these cells in a stepwise fashion before they become tumorigenic in nude mice. In the present study we used cDNA expression arrays to screen differentially expressed genes among the tumorigenic cells. A total of 15 genes were identified, 11 of which were further confirmed by northern blot. Expression levels of these genes were then determined among transformed BEP2D cells at different stages of the neoplastic process, including non-tumorigenic cells that were resistant to serum-induced terminal differentiation, early and late passage transformed BEP2D cells, five representative tumor cell lines and fused tumorigenic–control cell lines which were no longer tumorigenic. A consistent 2- to 3-fold down-regulation of the DCC (deleted in colon cancer), Ku70 and heat shock protein 27 genes were detected in all the independently generated tumor cell lines while expression levels in early transformants as well as in the fusion cell lines remained normal. In contrast, all the tumor cell lines examined demonstrated 2- to 4-fold overexpression of the insulin receptor and its signal transduction genes. Differential expression of these genes was completely restored in the fusion cell lines examined. No alteration in c-jun or EGF receptor expression was found in any of the cell lines. Our data suggest that activation of the insulin receptor pathway and inactivation of DCC and Ku70 may cooperate in malignant transformation of BEP2D cells induced by asbestos.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0143-3334, 1460-2180eISSN: 1460-2180DOI: 10.1093/carcin/21.11.2005Titel-ID: cdi_proquest_miscellaneous_17733632
- Format
- –
- Schlagworte
- activator protein-1, Animals, Antigens, Nuclear, AP-1, Asbestos, Serpentine - adverse effects, Biological and medical sciences, Blotting, Northern, Bronchi - drug effects, Bronchi - pathology, Bronchi - virology, Carcinogenesis, carcinogens and anticarcinogens, Cell Adhesion Molecules - biosynthesis, Cell Adhesion Molecules - genetics, Cell Fusion, Cell Line, Transformed, Cell physiology, Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes, Cell Transformation, Neoplastic - drug effects, Cell Transformation, Neoplastic - genetics, Cell Transformation, Neoplastic - metabolism, Chemical agents, DCC, DCC Receptor, deleted in colon cancer, DNA Helicases, DNA-Activated Protein Kinase, DNA-Binding Proteins - biosynthesis, DNA-Binding Proteins - genetics, DNA-dependent protein kinase, DNA-PK, Down-Regulation, Epithelial Cells - drug effects, Epithelial Cells - pathology, Epithelial Cells - virology, ERK, extracellular signal-related kinases, Fundamental and applied biological sciences. Psychology, G3PDH, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, glyceraldehyde 3-phosphate dehydrogenase, heat shock protein 27, HSP27, Humans, Ku Autoantigen, Lung Neoplasms - etiology, Lung Neoplasms - genetics, Lung Neoplasms - metabolism, Male, MAPK, Medical sciences, Mice, Mice, Nude, mitogen-activated protein kinase, Molecular and cellular biology, NFκB, nuclear factor κB, Nuclear Proteins - biosynthesis, Nuclear Proteins - genetics, Oligonucleotide Array Sequence Analysis, Papillomaviridae, Phenotype, Protein-Serine-Threonine Kinases - biosynthesis, Protein-Serine-Threonine Kinases - genetics, receptor tyrosine kinases, Receptor, Insulin - biosynthesis, Receptor, Insulin - genetics, Receptors, Cell Surface, RTK, simian virus 40, SV40, Tumor Suppressor Proteins, Tumors