Details

Autor(en) / Beteiligte

• Jiménez-Pardo, Isabel
• González-Pastor, Rebeca
• Lancelot, Alexandre
• Claveria-Gimeno, Rafael
• Velázquez-Campoy, Adrián
• Abian, Olga
• Ros, M. Blanca
• Sierra, Teresa

Titel

Shell Cross-Linked Polymeric Micelles as Camptothecin Nanocarriers for Anti-HCV Therapy

Ist Teil von

• Macromolecular bioscience, 2015-10, Vol.15 (10), p.1381-1391

Ort / Verlag

Germany: Blackwell Publishing Ltd

Erscheinungsjahr

2015

Quelle

Wiley-Blackwell Full Collection

Beschreibungen/Notizen

• A suitable carrier for camptothecin to act as therapy against the hepatitis C virus is presented. The carrier relies on an amphiphilic hybrid dendritic–linear–dendritic block copolymer, derived from pluronic F127 and bis‐MPA dendrons, that forms micelles in aqueous solution. The dendrons admit the incorporation of multiple photoreactive groups that allow the clean and effective preparation of covalently cross‐linked polymeric micelles (CLPM), susceptible of loading hydrophilic and lipophilic molecules. Cell‐uptake experiments using a newly designed fluorophore, derived from rhodamine B, demonstrate that the carrier favors the accumulation of its cargo within the cell. Furthermore, loaded with camptothecin, it is efficient in fighting against the hepatitis C virus while shows lower cytotoxicity than the free drug. Shell photocross‐linked polymeric micelles transport efficiently camptothecin into hepatitis C infected cells and show lower toxicity than the free drug. A design based on a hybrid dendritic–linear–dendritic block copolymer yields a macromer that permit effective preparation of stable carriers. An amphiphilic fluorescent probe proves the internalization of the carriers within the cells.