Details

Autor(en) / Beteiligte

• Shen, Yanting
• Tian, Fei
• Chen, Zhenzhu
• Li, Rui
• Ge, Qinyu
• Lu, Zuhong

Titel

Amplification-based method for microRNA detection

Ist Teil von

• Biosensors & bioelectronics, 2015-09, Vol.71, p.322-331

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Over the last two decades, the study of miRNAs has attracted tremendous attention since they regulate gene expression post-transcriptionally and have been demonstrated to be dysregulated in many diseases. Detection methods with higher sensitivity, specificity and selectivity between precursors and mature microRNAs are urgently needed and widely studied. This review gave an overview of the amplification-based technologies including traditional methods, current modified methods and the cross-platforms of them combined with other techniques. Many progresses were found in the modified amplification-based microRNA detection methods, while traditional platforms could not be replaced until now. Several sample-specific normalizers had been validated, suggesting that the different normalizers should be established for different sample types and the combination of several normalizers might be more appropriate than a single universal normalizer. This systematic overview would be useful to provide comprehensive information for subsequent related studies and could reduce the un-necessary repetition in the future. •The amplification-based technology with significant advantage in higher sensitivity is a key component of miRNA studies.•Advanced amplification-based methods cannot replace traditional ones to be conventionally used due to the lack of enough verification.•Several sample-specific normalizers have been validated, suggesting that a single universal normalizer for all sample types is unlikely to exist, yet the use of different combinations of several normalizers for different sample types may be more appropriate.