Details

Autor(en) / Beteiligte

• Li, Guanying
• Lin, Qian
• Sun, Lingli
• Feng, Changsheng
• Zhang, Pingyu
• Yu, Bole
• Chen, Yu
• Wen, Ya
• Wang, Hui
• Ji, Liangnian
• Chao, Hui

Titel

A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo

Ist Teil von

• Biomaterials, 2015-06, Vol.53, p.285-295

Ort / Verlag

Netherlands: Elsevier Ltd

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Abstract Endogenous hypochlorite ion (ClO− ) is a highly reactive oxygen species (ROS) that is produced from hydrogen peroxide and chloride ions catalyzed by myeloperoxidase (MPO). And mitochondrion is one of the major sources of ROS including ClO− . In the present work, a two-photon phosphorescent probe for ClO− in mitochondria was developed. An iridium(III) complex bearing a diaminomaleonitrile group as ClO− reactive moiety specifically responded to ClO− over other ions and ROSs. When the probe was reacted with ClO− to form an oxidized carboxylate product, a significant enhancement in phosphorescence intensity was observed under one-photon (402 nm) and two-photon (750 nm) excitation, with a two-photon absorption cross-section of 78.1 GM at 750 nm. More importantly, ICP-MS results and cellular images co-stained with Mito-tracker Green demonstrated that this probe possessed high specificity for mitochondria. This probe was applied in the one- and two-photon imaging of ClO− in vitro and in vivo. The results suggested endotoxin lipopolysaccharide (LPS) induced ClO− mostly generated in the liver of zebrafish.