Details

Autor(en) / Beteiligte

• Kondo, Jiro
• Tada, Yoshinari
• Dairaku, Takenori
• Saneyoshi, Hisao
• Okamoto, Itaru
• Tanaka, Yoshiyuki
• Ono, Akira

Titel

High-Resolution Crystal Structure of a Silver(I)-RNA Hybrid Duplex Containing Watson-Crick-like CSilver(I)C Metallo-Base Pairs

Ist Teil von

• Angewandte Chemie (International ed.), 2015-11, Vol.54 (45), p.13323-13326

Auflage

International ed. in English

Ort / Verlag

Weinheim: WILEY-VCH Verlag

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Metallo‐base pairs have been extensively studied for applications in nucleic acid‐based nanodevices and genetic code expansion. Metallo‐base pairs composed of natural nucleobases are attractive because nanodevices containing natural metallo‐base pairs can be easily prepared from commercially available sources. Previously, we have reported a crystal structure of a DNA duplex containing THgIIT base pairs. Herein, we have determined a high‐resolution crystal structure of the second natural metallo‐base pair between pyrimidine bases CAgIC formed in an RNA duplex. One AgI occupies the center between two cytosines and forms a CAgIC base pair through N3AgIN3 linear coordination. The CAgIC base pair formation does not disturb the standard A‐form conformation of RNA. Since the CAgIC base pair is structurally similar to the canonical Watson–Crick base pairs, it can be a useful building block for structure‐based design and fabrication of nucleic acid‐based nanodevices. The high‐resolution structures of CAgIC base pairs in A‐form RNA duplexes have been solved. Structural information on the present metallo‐base pair together with the previously reported THgIIT base pair widely open the possibility of structure‐based design of nucleic‐acid‐based nanodevices containing the natural metallo‐base pairs.