Molecular cell, 2000-09, Vol.6 (3), p.637-648
2000
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Details
- Autor(en) / Beteiligte
- Titel
- Structure and Function of Cdc6/Cdc18: Implications for Origin Recognition and Checkpoint Control
- Ist Teil von
- Molecular cell, 2000-09, Vol.6 (3), p.637-648
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2000
- Quelle
- EZB-FREE-00999 freely available EZB journals
- Beschreibungen/Notizen
- Cdc6/Cdc18 is a conserved and essential component of prereplication complexes. The 2.0 Å crystal structure of an archaeal Cdc6 ortholog, in conjunction with a mutational analysis of the homologous Cdc18 protein from Schizosaccharomyces pombe, reveals novel aspects of Cdc6/Cdc18 function. Two domains of Cdc6 form an AAA +-type nucleotide binding fold that is observed bound to Mg•ADP. A third domain adopts a winged-helix fold similar to known DNA binding modules. Sequence comparisons show that the winged-helix domain is conserved in Orc1, and mutagenesis data demonstrate that this region of Cdc6/Cdc18 is required for function in vivo. Additional mutational analyses suggest that nucleotide binding and/or hydrolysis by Cdc6/Cdc18 is required not only for progression through S phase, but also for maintenance of checkpoint control during S phase.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1097-2765eISSN: 1097-4164DOI: 10.1016/S1097-2765(00)00062-9Titel-ID: cdi_proquest_miscellaneous_17697827
- Format
- –
- Schlagworte
- Alleles, Cdc18 protein, Cdc6 protein, Cell Cycle Proteins - chemistry, Cell Cycle Proteins - genetics, Cell Cycle Proteins - metabolism, Cloning, Molecular, Crystallography, DNA Mutational Analysis, DNA-Binding Proteins - genetics, Fungal Proteins - chemistry, Fungal Proteins - genetics, Fungal Proteins - metabolism, Gene Expression Regulation, Fungal, Histones - chemistry, Histones - genetics, Histones - metabolism, Molecular Sequence Data, Mutation - physiology, Nucleotides - metabolism, Origin Recognition Complex, Phenotype, Protein Structure, Secondary, Protein Structure, Tertiary, Replication Origin - physiology, S Phase - physiology, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe, Schizosaccharomyces pombe Proteins, Sequence Homology, Amino Acid, Structure-Activity Relationship