Details

Autor(en) / Beteiligte

• Appolinario, Luciana R
• Tschoeke, Diogo A
• Rua, Cintia P. J
• Venas, Tainá
• Campeão, Mariana E
• Amaral, Gilda R. S
• Leomil, Luciana
• de Oliveira, Louisi
• Vieira, Verônica Viana
• Otsuki, Koko
• Swings, Jean
• Thompson, Fabiano L
• Thompson, Cristiane C

Titel

Description of Endozoicomonas arenosclerae sp. nov. using a genomic taxonomy approach

Ist Teil von

• Antonie van Leeuwenhoek, 2016-03, Vol.109 (3), p.431-438

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• The taxonomic position of strains Ab112ᵀ (CBAS 572ᵀ) and Ab227_MC (CBAS 573) was evaluated by means of genomic taxonomy. These isolates represent the dominant flora cultured from the healthy marine sponge Arenosclera brasiliensis, endemic to Rio de Janeiro. Strains CBAS 572ᵀ and CBAS 573 shared >98 % 16S rRNA sequence identity with Endozoicomonas numazuensis and Endozoicomonas montiporae. In silico DNA–DNA Hybridization, i.e. genome-to-genome distance (GGD), amino acid identity (AAI) and average nucleotide identity (ANI) further showed that these strains had <70 %, at maximum 71.1 and 78 % of identity, respectively, to their closest neighbours E. numazuensis and E. montiporae. The DNA G+C content of CBAS 572ᵀ and CBAS 573 were 47.6 and 47.7 mol%, respectively. Phenotypic and chemotaxonomic features also allowed a separation from the type strains of their phylogenetic neighbours. Useful phenotypic features for discriminating CBAS 572ᵀ and CBAS 573 from E. numazuensis and E. montiporae species include C8 esterase, N-acetyl-β-glucosaminidase, citric acid, uridine and siderophore. The species Endozoicomonas arenosclerae sp. nov. is proposed to harbour the new isolates. The type strain is CBAS 572ᵀ (=Ab112ᵀ).