Details

Autor(en) / Beteiligte

• Cousin, H
• Alfandari, D

Titel

A PTP-PEST-like protein affects alpha 5 beta 1-integrin-dependent matrix assembly, cell adhesion, and migration in Xenopus gastrula

Ist Teil von

• Developmental biology, 2004-01, Vol.265 (2), p.416-432

Erscheinungsjahr

2004

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• During amphibian gastrulation, mesodermal cell movements depend on both cell-cell and cell-matrix interactions. Ectodermal cells from the blastocoel roof use alpha 5 beta 1 integrins to assemble a fibronectin-rich extracellular matrix on which mesodermal cells migrate using the same alpha 5 beta 1 integrin. In this report, we show that the tyrosine phosphatase xPTP-PESTr can prevent fibronectin fibril formation when overexpressed in ectodermal cells resulting in delayed gastrulation. In addition, isolated ectodermal cells overexpressing xPTP-PESTr are able to spread on fibronectin using the alpha 5 beta 1 integrin in the absence of activin-A induction and before the onset of gastrulation. We further show that while the inhibition of fibrillogenesis depends on the phosphatase activity of xPTP-PESTr, induction of cell spreading does not. Finally, while cell spreading is usually associated with cell migration, xPTP-PESTr promotes ectodermal cell spreading on fibronectin but also reduces cell migration in response to activin-A, suggesting an adverse effect on cell translocation. We propose that xPTP-PESTr overexpression adversely affect cell migration by preventing de-adhesion of cells from the substrate.