Details

Autor(en) / Beteiligte

• Mirković, Bojana, PhD
• Lavelle, Gillian M., BSc
• Azim, Ahmed Abdul, MB, BCh, BAO
• Helma, Kristine, MD
• Gargoum, Fatma S., MD
• Molloy, Kevin, MB, BCh, BAO
• Gernez, Yael, MD, PhD
• Dunne, Katie, PhD
• Renwick, Julie, PhD
• Murphy, Philip, MD
• Moss, Richard B., MD
• Greene, Catherine M., PhD
• Gunaratnam, Cedric, MB
• Chotirmall, Sanjay H., MD, PhD
• McElvaney, Noel G., MD

Titel

The basophil surface marker CD203c identifies Aspergillus species sensitization in patients with cystic fibrosis

Ist Teil von

• Journal of allergy and clinical immunology, 2016-02, Vol.137 (2), p.436-443.e9

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2016

Quelle

ScienceDirect Pay Per View(PPV) Titles

Beschreibungen/Notizen

• Background Colonization by Aspergillus fumigatus in patients with cystic fibrosis (CF) can cause A fumigatus sensitization and/or allergic bronchopulmonary aspergillosis (ABPA), which affects pulmonary function and clinical outcomes. Recent studies show that specific allergens upregulate the surface-expressed basophil marker CD203c in sensitized subjects, a response that can be readily measured by using flow cytometry. Objective We sought to identify A fumigatus sensitization in patients with CF by using the basophil activation test (BAT). Methods Patients with CF attending Beaumont Hospital were screened for study inclusion. BAT was used to identify A fumigatus sensitization. Serologic (total and A fumigatus –specific IgE), pulmonary function, and body mass index measurements were performed. Results The BAT discriminates A fumigatus –sensitized from nonsensitized patients with CF. Persistent isolation of A fumigatus in sputum is a significant risk factor for A fumigatus sensitization. Levels of the A fumigatus –stimulated basophil activation marker CD203c inversely correlated with pulmonary function and body mass index in A fumigatus –sensitized but not nonsensitized patients with CF. Total and A fumigatus –specific IgE, but not IgG, levels are increased in A fumigatus –sensitized patients with CF and ABPA when compared with those in A fumigatus –sensitized and nonsensitized patients with CF without ABPA. Itraconazole treatment did not affect A fumigatus sensitization. Conclusion Combining the BAT with routine serologic testing allows classification of patients with CF into 3 groups: nonsensitized, A fumigatus –sensitized, and ABPA. Accurate and prompt identification of A fumigatus –associated clinical status might allow early and targeted therapeutic intervention, potentially improving clinical outcomes.