Details

Autor(en) / Beteiligte

• Clemente-Ruiz, Marta
• Murillo-Maldonado, Juan M.
• Benhra, Najate
• Barrio, Lara
• Pérez, Lidia
• Quiroga, Gonzalo
• Nebreda, Angel R.
• Milán, Marco

Titel

Gene Dosage Imbalance Contributes to Chromosomal Instability-Induced Tumorigenesis

Ist Teil von

• Developmental cell, 2016-02, Vol.36 (3), p.290-302

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2016

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Chromosomal instability (CIN) is thought to be a source of mutability in cancer. However, CIN often results in aneuploidy, which compromises cell fitness. Here, we used the dosage compensation mechanism (DCM) of Drosophila to demonstrate that chromosome-wide gene dosage imbalance contributes to the deleterious effects of CIN-induced aneuploidy and its pro-tumorigenic action. We present evidence that resetting of the DCM counterbalances the damaging effects caused by CIN-induced changes in X chromosome number. Importantly, interfering with the DCM suffices to mimic the cellular effects of aneuploidy in terms of reactive oxygen species (ROS) production, JNK-dependent cell death, and tumorigenesis upon apoptosis inhibition. We unveil a role of ROS in JNK activation and a variety of cellular and tissue-wide mechanisms that buffer the deleterious effects of CIN, including DNA-damage repair, activation of the p38 pathway, and cytokine induction to promote compensatory proliferation. Our data reveal the existence of robust compensatory mechanisms that counteract CIN-induced cell death and tumorigenesis. •Chromosome-wide gene dosage imbalances contribute to aneuploidy-induced cell death•Chromosome-wide gene dosage imbalances induce a tumorigenic behavior•The DDR pathway reduces the levels of CIN-induced aneuploidy and tumorigenesis•Gene dosage imbalances induce ROS, which contribute to CIN-induced tumorigenesis Many solid tumors exhibit chromosomal instability (CIN) and contain an abnormal number of chromosomes or chromosomal parts. Clemente-Ruiz et al. identify multiple mechanisms that buffer the deleterious effects of CIN in proliferating epithelial tissues and demonstrate that chromosome-wide gene dosage imbalances contribute to the induction of cell death and tumorigenesis.