Details
- Autor(en) / Beteiligte
- Titel
- CXCL16 induces angiogenesis in autocrine signaling pathway involving hypoxia-inducible factor 1α in human umbilical vein endothelial cells
- Ist Teil von
- Oncology reports, 2016-03, Vol.35 (3), p.1557-1565
- Ort / Verlag
- Greece: D.A. Spandidos
- Erscheinungsjahr
- 2016
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Chemokine (C-X-C motif) ligand 16 (CXCL16) is a new angiogenic factor inducing angiogenesis via extracellular signal-regulated kinases pathway. To further understand the molecular mechanism underlying CXCL16-induced angiogenesis, we explored involvement of other relevant pathways in CXCL16-induced angiogenesis. In the present study, we investigated the mechanisms underlying CXCL16-induced angiogenesis in human umbilical vein endothelial cells (HUVECs). CXCL16 promoted HUVEC proliferation, tube formation and migration. Enzyme-linked immunosorbent assay revealed that CXCL16 induced vascular endothelial growth factor secretion from HUVECs. Western blot analysis showed that CXCL16 increased the level of hypoxia-inducible factor 1α, p-extracellular signal-regulated kinases (ERK), p-p38 and p-Akt dose- and time-dependently. ERK-, p38- and Akt-selective inhibitors significantly suppressed HUVEC proliferation, migration, tube formation and hypoxia-inducible factor 1α (HIF-1α) expression induced by CXCL16. Furthermore, CXCL16 peptides induced CXCL16 secretion via ERK, p38 and Akt pathways, which was suppressed by HIF-1α-selective inhibitor PX12. Our data suggest that CXCL16 induces angiogenesis in autocrine manner via ERK, Akt, p38 pathways and HIF-1α modulation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1021-335XeISSN: 1791-2431DOI: 10.3892/or.2015.4520Titel-ID: cdi_proquest_miscellaneous_1764698907
- Format
- –
- Schlagworte
- Akt pathway, Angiogenesis, autocrine, Autocrine Communication - genetics, Cancer, Cell adhesion & migration, Cell growth, Cell Movement - genetics, Cell Proliferation - genetics, Cellular signal transduction, Chemokine CXCL16, Chemokines, Chemokines, CXC - biosynthesis, Chemokines, CXC - genetics, CXCL16, ERK pathway, Gene Expression Regulation, Neoplastic, Genetic aspects, Growth factors, Health aspects, Human Umbilical Vein Endothelial Cells - metabolism, Human Umbilical Vein Endothelial Cells - pathology, Humans, Hypoxia, Hypoxia-Inducible Factor 1, alpha Subunit - biosynthesis, Hypoxia-Inducible Factor 1, alpha Subunit - genetics, hypoxia-inducible factor 1α, Kinases, MAP Kinase Signaling System - genetics, Neovascularization, Neovascularization, Pathologic - genetics, Neovascularization, Pathologic - pathology, p38 pathway, Polypeptides, Properties, Proteins, Proto-Oncogene Proteins c-akt - genetics, Receptors, Scavenger - biosynthesis, Receptors, Scavenger - genetics, Transcription factors, Vascular Endothelial Growth Factor A - biosynthesis, Vascular Endothelial Growth Factor A - genetics