Details

Autor(en) / Beteiligte

• Díaz-Quiñonez, José Alberto
• Escobar-Escamilla, Noé
• Ortíz-Alcántara, Joanna
• Vázquez-Pichardo, Mauricio
• de la Luz Torres-Rodríguez, María
• Nuñez-León, Alma
• Torres-Longoria, Belem
• López-Martínez, Irma
• Ruiz-Matus, Cuitláhuac
• Kuri-Morales, Pablo
• Ramírez-González, José Ernesto

Titel

Identification of Asian genotype of chikungunya virus isolated in Mexico

Ist Teil von

• Virus genes, 2016-02, Vol.52 (1), p.127-129

Ort / Verlag

New York: Springer US

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• We identified 25 autochthonous chikungunya virus cases in Mexico, initially detected by RT-PCR targeting the E1 gene and propagated in C6/36 Aedes albopictus cells, in 2014. To determine the type of virus found, in a previous report, the genomes of 2 CHIKV strains were fully sequenced. Genome sequence analysis revealed that these isolates from Mexico belonged to the Asian genotype, and a phylogenetic association with the circulating strain in the British Virgin Islands was also established in the same year. This was further supported by changes in specific amino acids, E2-V368A and 6K-L20M. For these reasons, it can be inferred that the route of virus entry to Mexico was held across the countries in the Caribbean and Central America. The presence of E1-A226V mutation associated with more efficient replication in the salivary gland of the A. albopictus mosquito was not observed. Interestingly, a newly acquired NSP4-S399C mutation was observed; however, the significance of changes in amino acid found in non-structural proteins in autochthonous strains remains to be elucidated.