Details

Autor(en) / Beteiligte

• Hsu, Ssucheng J
• Erickson, Robert P
• Zhang, Jingsong
• Garver, William S
• Heidenreich, Randall A

Titel

Fine linkage and physical mapping suggests cross-over suppression with a retroposon insertion at the npc1 mutation

Ist Teil von

• Mammalian genome, 2000-09, Vol.11 (9), p.774-778

Ort / Verlag

United States: Springer-Verlag

Erscheinungsjahr

2000

Quelle

SpringerLINK Contemporary (Konsortium Baden-Württemberg)

Beschreibungen/Notizen

• Mouse Niemann-Pick disease type C1 (npc1), formerly designated spm (sphingomyelinosis), is an autosomal recessive lipid storage disorder. We generated a high-resolution linkage map in the 2.24-cM npc1 critical region by typing eight polymorphic markers in 2322 meioses (948 of these were previously reported). A minimal set of overlapping yeast artificial chromosomes (YACs) had previously been assembled (Hsu and Erickson 2000). The YAC 313-B-8, which covered this whole region, has been used to construct cosmid libraries. Three cosmid contigs were built, and one of them contained the npc1 locus. Two (CA)ₙ microsatellites were identified, and the one new one was characterized, from the YAC-derived cosmids. The most proximal cosmid contig overlaps with markers near twirler (Tw). Both the physical map and genetic linkage map have been integrated to study the recombination frequencies in this particular region of the mouse genome, and recombination suppression due to the heterozygous insertion of DNA was suggested.