FEBS letters, 2015-12, Vol.589 (24), p.3952-3958
2015
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- Autor(en) / Beteiligte
- Titel
- Clostridium difficile secreted Pro-Pro endopeptidase PPEP-1 (ZMP1/CD2830) modulates adhesion through cleavage of the collagen binding protein CD2831
- Ist Teil von
- FEBS letters, 2015-12, Vol.589 (24), p.3952-3958
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- •C. difficile Pro-Pro endopeptidase (PPEP-1) has a strict preference for cleaving between two prolines.•PPEP-1 has a preference for an Asn at the P2, and a Val, Ile or Leu at the P3 position.•PPEP-1 knockout cells retain the collagen binding protein CD2831 on the cell surface.•PPEP-1 knockout cells show higher affinity for collagen type I.•PPEP-1 knockout cells show attenuated virulence in a hamster infection model. The Clostridium difficile cd2830 gene product is a secreted metalloprotease, named Pro-Pro endopeptidase (PPEP-1). PPEP-1 cleaves C. difficile cell surface proteins (e.g. CD2831). Here, we confirmed that PPEP-1 has a unique preference for prolines surrounding the scissile bond. Moreover, we show that it exhibits a high preference for an asparagine at the P2 position and hydrophobic residues at the P3 position. Using a PPEP-1 knockout C. difficile strain, we demonstrate that the removal of the collagen binding protein CD2831 is fully attributable to PPEP-1 activity. The PPEP-1 knockout strain demonstrated higher affinity for collagen type I with attenuated virulence in hamsters.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/j.febslet.2015.10.027Titel-ID: cdi_proquest_miscellaneous_1762367829
- Format
- –
- Schlagworte
- 4-(dimethylaminoazo)benzene-4-carboxylic acid, 5-[(2 aminoethyl)amino]naphthalene-1-sulfonic acid, Adhesion, Amino Acid Motifs, Animals, Bacterial Adhesion, Bacterial Proteins - chemistry, Bacterial Proteins - metabolism, Bacterial virulence, Clostridioides difficile - enzymology, Clostridioides difficile - pathogenicity, Clostridioides difficile - physiology, Clostridium difficile, Collagen binding, Collagen Type I - metabolism, Dabcyl, EDANS, Female, Gene Knockout Techniques, Hydrophobic and Hydrophilic Interactions, mass spectrometry, Membrane Proteins - chemistry, Membrane Proteins - genetics, Membrane Proteins - metabolism, Mesocricetus, Metalloendopeptidases - genetics, Metalloendopeptidases - metabolism, Motility, MS/MS, Mutation, Peptide Fragments - chemistry, Peptide Fragments - genetics, Peptide Fragments - metabolism, PPEP-1, Pro-Pro endopeptidase 1, Protein Interaction Domains and Motifs, Proteolysis, Rats, Recombinant Fusion Proteins - chemistry, Recombinant Fusion Proteins - metabolism, retention time, Secreted protease, Substrate Specificity, tandem mass spectrometry, Virulence