Details

Autor(en) / Beteiligte

• Xiao, Xilin
• Zhu, Guizhi
• Liao, Lifu
• Liu, Bo
• Yuan, Yali
• Wang, Yongsheng
• He, Jun
• He, Bo
• Wu, Yimou

Titel

A square wave voltammetric method for the detection of microorganism populations using a MWNT-modified glassy carbon electrode

Ist Teil von

• Electrochimica acta, 2012-07, Vol.74, p.105-110

Ort / Verlag

Kidlington: Elsevier Ltd

Erscheinungsjahr

2012

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A novel method for determination of trace amounts of microorganism populations in solution was developed by using a multiwalled carbon nanotube (MWNT) modified glassy carbon electrode and square wave voltammetry (SWV). The simultaneous combination of MWNT and SWV allowed the electrochemical signal of electro-active materials in Escherichia coli O157:H7 (E. coli) to be dramatically amplified. Compared with a bare glassy carbon electrode, the MWNT-modified glassy carbon electrode showed catalytic properties in the oxidation of electro-active materials on cell surfaces. Moreover, SWV was proved to be more sensitive than cyclic voltammetry (CV) for investigation of the electrochemical behavior of cells. In this paper, a linear relationship was obtained between the SWV peak current and the cell concentration in the range 2×102–2×108cellmL−1 with a detection limit of 2×102cellmL−1. The effect of antibiotic drug Gentamycin Sulfate injection (GSI) on the growth of E. coli was also investigated.