Molecular and cellular endocrinology, 2013-08, Vol.375 (1-2), p.79-88
2013
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- Autor(en) / Beteiligte
- Titel
- Expression and functional activity of PACAP and its receptors on cumulus cells: Effects on oocyte maturation
- Ist Teil von
- Molecular and cellular endocrinology, 2013-08, Vol.375 (1-2), p.79-88
- Ort / Verlag
- Ireland: Elsevier Ireland Ltd
- Erscheinungsjahr
- 2013
- Quelle
- ScienceDirect
- Beschreibungen/Notizen
- •PACAP and its receptor PAC1-R are transiently expressed in mouse cumulus cells after the LH surge.•PACAP has beneficial effects on both nuclear and cytoplasmic oocyte maturation.•PACAP supplementation might reconstruct a more physiological environment for in vitro maturation.•PACAP supplementation might be beneficial for correct preimplantation development. Pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptor PAC1-R (PACAP type 1 receptor) are transiently expressed in granulosa cells (GCs) of mouse preovulatory follicles and affect several parameters associated with the ovulatory process. We investigated the expression of PACAP and its receptors in cumulus cells (CCs) after the LH surge and their role on cumulus expansion/apoptosis and oocyte maturation. PACAP and PAC1-R expression increased in CCs isolated at different times after treatment with human chorionic gonadotropin (hCG). Moreover, PACAP was able to reverse the inhibition of oocyte meiotic maturation caused by hypoxantine in cumulus cell-oocyte complexes (COCs) and efficiently promoted male pronuclear formation after fertilisation. PACAP was also able to induce cumulus expansion and prevent CC apoptosis. Our results demonstrated the induction of PACAP and its receptors in CCs by LH and EGF, suggesting that PACAP may play a significant role in the complex interactions of gonadotropin and growth factors during ovulation and fertilisation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0303-7207eISSN: 1872-8057DOI: 10.1016/j.mce.2013.05.006Titel-ID: cdi_proquest_miscellaneous_1762046230
- Format
- –
- Schlagworte
- 8-Bromo Cyclic Adenosine Monophosphate - pharmacology, Amphiregulin, Animals, Apoptosis, Cell Nucleus - physiology, Cell Proliferation, Cells, Cultured, Chorionic Gonadotropin - physiology, Cumulus Cells - drug effects, Cumulus Cells - metabolism, Cumulus expansion, Cytoplasm - metabolism, EGF Family of Proteins, Endocrinology, Epidermal Growth Factor - physiology, Female, Fertilization in Vitro, Follicle Stimulating Hormone - physiology, Formations, Glycoproteins - genetics, Glycoproteins - metabolism, granulosa cells, Growth factors, Human, human chorionic gonadotropin, Humans, Intercellular Signaling Peptides and Proteins - genetics, Intercellular Signaling Peptides and Proteins - metabolism, luteinizing hormone, Male, Males, Maturation, Mice, Oocyte maturation, oocytes, Oocytes - physiology, ovulation, Ovulation - metabolism, PACAP, Pituitary Adenylate Cyclase-Activating Polypeptide - genetics, Pituitary Adenylate Cyclase-Activating Polypeptide - metabolism, polypeptides, Receptors, Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - genetics, Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - metabolism, Receptors, Vasoactive Intestinal Peptide, Type II - genetics, Receptors, Vasoactive Intestinal Peptide, Type II - metabolism, RNA, Messenger - genetics, RNA, Messenger - metabolism, Sperm Head - physiology