Details

Autor(en) / Beteiligte

• Patel, Sahishnu
• Chueng, Sy-Tsong Dean
• Yin, Perry T.
• Dardir, Kholud
• Song, Zhichao
• Pasquale, Nicholas
• Kwan, Kelvin
• Sugiyama, Hiroshi
• Lee, Ki-Bum

Titel

Induction of Stem-Cell-Derived Functional Neurons by NanoScript-Based Gene Repression

Ist Teil von

• Angewandte Chemie, 2015-10, Vol.127 (41), p.12151-12156

Ort / Verlag

Weinheim: WILEY-VCH Verlag

Erscheinungsjahr

2015

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Even though gene repression is a powerful approach to exogenously regulate cellular behavior, developing a platform to effectively repress targeted genes, especially for stem‐cell applications, remains elusive. Herein, we introduce a nanomaterial‐based platform that is capable of mimicking the function of transcription repressor proteins to downregulate gene expression at the transcriptional level for enhancing stem‐cell differentiation. We developed the “NanoScript” platform by integrating multiple gene repression molecules with a nanoparticle. First, we show a proof‐of‐concept demonstration using a GFP‐specific NanoScript to knockdown GFP expression in neural stem cells (NSCs‐GFP). Then, we show that a Sox9‐specific NanoScript can repress Sox9 expression to initiate enhanced differentiation of NSCs into functional neurons. Overall, the tunable properties and gene‐knockdown capabilities of NanoScript enables its utilization for gene‐repression applications in stem cell biology. Das Nanopartikel „NanoScript”︁ ist die Schlüsselkomponente eines nichtviralen Systems zur Ausschaltung der transkriptionellen Genexpression in Stammzellen. NanoScript kann mit spezifischen niedermolekularen Substanzen funktionalisiert werden, um GFP in GFP‐markierten neuralen Stammzellen (NSCs) effektiv auszuschalten und die Sox9‐Expression in NSCs zu unterdrücken.