Lab on a chip, 2015-05, Vol.15 (10), p.2192-2200
2015
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Automated co-culture system for spatiotemporal analysis of cell-to-cell communication
- Ist Teil von
- Lab on a chip, 2015-05, Vol.15 (10), p.2192-2200
- Ort / Verlag
- England
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We present a microfluidic co-culture system that generates localized and precisely formulated immune signals among a population of cells, enabling spatiotemporal analysis of paracrine signal transmission between different cell types. The automated system allows us to create temporally modulated chemical inputs that can be delivered to single signal-transmitting and receiving cells in a highly controlled way. Using this system we stimulated a single macrophage with brief pulses of bacterial LPS and observed the macrophage transmitted TNF signal propagating in a population of fibroblasts via NF-κB activation. The signal receiving fibroblasts transformed the TNF signal into a spatiotemporally distributed NF-κB output, recapitulating the initiation of immune response to bacterial infection.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1473-0197eISSN: 1473-0189DOI: 10.1039/c5lc00182jTitel-ID: cdi_proquest_miscellaneous_1753524062
- Format
- –
- Schlagworte
- Animals, Automation, Bacteria, Cell Culture Techniques - instrumentation, Cell Culture Techniques - methods, Coculture Techniques - instrumentation, Coculture Techniques - methods, Communication systems, Control systems, Fibroblasts, Fibroblasts - cytology, Fibroblasts - immunology, Lipopolysaccharides - pharmacology, Macrophages, Mice, Mice, Knockout, Microfluidics, NF-kappa B - immunology, NIH 3T3 Cells, Paracrine Communication - drug effects, Receiving, Signal Transduction - drug effects, Signal transmission, Tumor Necrosis Factor-alpha - immunology