Biotechnology and bioengineering, 2011-10, Vol.108 (10), p.2261-2269
2011
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- Autor(en) / Beteiligte
- Titel
- Altered substrate specificity of the gluco-oligosaccharide oxidase from Acremonium strictum
- Ist Teil von
- Biotechnology and bioengineering, 2011-10, Vol.108 (10), p.2261-2269
- Ort / Verlag
- Hoboken: Wiley Subscription Services, Inc., A Wiley Company
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- A gluco‐oligosaccharide oxidase (GOOX) from Acremonium strictum type strain CBS 346.70 was cloned and expressed in Pichia pastoris. The recombinant protein, GOOX‐VN, contained fifteen amino acid substitutions compared with the previously reported A. strictum GOOX. These two enzymes share 97% sequence identity; however, only GOOX‐VN oxidized xylose, galactose, and N‐acetylglucosamine. Besides monosaccharides, GOOX‐VN oxidized xylo‐oligosaccharides, including xylobiose and xylotriose with similar catalytic efficiency as for cello‐oligosaccharides. Of three mutant enzymes that were created in GOOX‐VN to improve substrate specificity, Y300A and Y300N doubled kcat values for monosaccharide and oligosaccharide substrates. With this novel substrate specificity, GOOX‐VN and its variants are particularly valuable for oxidative modification of cello‐ and xylo‐oligosaccharides. Biotechnol. Bioeng. 2011;108: 2261–2269. © 2011 Wiley Periodicals, Inc.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-3592, 1097-0290eISSN: 1097-0290DOI: 10.1002/bit.23149Titel-ID: cdi_proquest_miscellaneous_1753506471
- Format
- –
- Schlagworte
- Acremonium, Acremonium - enzymology, Acremonium - genetics, Acremonium strictum, Alcohol Oxidoreductases - chemistry, Alcohol Oxidoreductases - genetics, Alcohol Oxidoreductases - metabolism, Amino Acid Substitution, Amino acids, Biological and medical sciences, Biotechnology, carbohydrate docking, Cloning, Molecular, Enzymes, Fundamental and applied biological sciences. Psychology, Fungal Proteins - chemistry, Fungal Proteins - genetics, Fungal Proteins - metabolism, Galactose, Gene Expression, gluco-oligosaccharide oxidase, Methods. Procedures. Technologies, Monosaccharides, Mutation, Mutation, Missense, Oligosaccharides, Oxidase, Pichia, Pichia pastoris, Protein engineering, Proteins, Recombinant, substrate specificity, Substrate Specificity - genetics, Xylose