Details

Autor(en) / Beteiligte

• Contin, Mario
• Flor, Sabrina
• Martinefski, Manuela
• Lucangioli, Silvia
• Tripodi, Valeria

Titel

The use of coenzyme Q0 as a template in the development of a molecularly imprinted polymer for the selective recognition of coenzyme Q10

Ist Teil von

• Analytica chimica acta, 2014-01, Vol.807, p.67-74

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2014

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Scheme of the synthesis of CoQ10-MIP obtained by polymerisation of CoQ0, MAA and EGDMA. •The first development of a coenzyme Q0 imprinted polymer used as a specific sorbent in CoQ10 analysis of biological matrices.•The successful use of an analogue of the target analyte as template to avoid the interference due to template bleeding.•The easy, low cost and high reproducibility in the polymer preparation.•The better clean-up of the sample with respect to traditional methodologies.•The application of the procedure to a real sample. In this work, a novel molecularly imprinted polymer (MIP) for use as a solid phase extraction sorbent was developed for the determination of coenzyme Q10 (CoQ10) in liver extract. CoQ10 is an essential cofactor in mitochondrial oxidative phosphorylation and a powerful antioxidant agent found in low concentrations in biological samples. This fact and its high hydrophobicity make the analysis of CoQ10 technically challenging. Accordingly, a MIP was synthesised using coenzyme Q0 as the template, methacrylic acid as the functional monomer, acetonitrile as the porogen, ethylene glycol dimethacrylate as the crosslinker and benzoyl peroxide as the initiator. Various parameters affecting the polymer preparation and extraction efficiency were evaluated. Morphological characterisation of the MIP and its proper comparison with C18 as a sorbent in solid phase extraction were performed. The optimal conditions for the molecularly imprinted solid phase extraction (MISPE) consisted of 400μL of sample mixed with 30mg of MIP and 600μL of water to reach the optimum solution loading. The loading was followed by a washing step consisting of 1mL of a 1-propanol solution (1-propanol:water, 30:70,v/v) and elution with 1mL of 1-propanol. After clean-up, the CoQ10 in the samples was analysed by high performance liquid chromatography. The extraction recoveries were higher than 73.7% with good precision (3.6–8.3%). The limits of detection and quantification were 2.4 and 7.5μgg−1, respectively, and a linear range between 7.5 and 150μgg−1 of tissue was achieved. The new MISPE procedure provided a successful clean-up for the determination of CoQ10 in a complex matrix.