Details

Autor(en) / Beteiligte

• Liu, Ze-Hong
• Li, Jing
• Xia, Jing
• Jiang, Rong
• Zuo, Guo-Wei
• Li, Xiao-Peng
• Chen, Yi
• Xiong, Wei
• Chen, Di-Long

Titel

Ginsenoside 20(s)-Rh2 as potent natural histone deacetylase inhibitors suppressing the growth of human leukemia cells

Ist Teil von

• Chemico-biological interactions, 2015-12, Vol.242, p.227-234

Ort / Verlag

Ireland: Elsevier B.V

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Activation and abnormal expression of histone deacetylase (HDAC) which is important target for cancer therapeutics are related to the occurrence of human leukemia. 20(s)-Ginsenoside Rh2 (20(s)-Rh2) may be a potential HDAC inhibitor (HDACi) of leukemia, but the mechanism has not been reported. The cell proliferation and apoptosis was assessed in cultured K562 and KG-1α cells. The protein expression was measured with immunoblotting. The activities of HDAC and histone acetyltransferase (HAT) were measured with BCA. In vivo experiments were performed on naked mice carrying K562 cells for assessment of tumor growth, apoptosis, protein expression, and HDAC/HAT activities. 20(s)-Rh2 effectively induced cell cycle arrest at G0/G1 phase and apoptosis in K562 and KG1-α cells, decreased the levels of proteins associated with cell proliferation (Cyclin D1, Bcl-2, ERK, p-ERK) and activated pro-apoptotic proteins (Bax, cleaved Caspase-3, p38, p-p38, JNK, p-JNK). 20(s)-Rh2 down-regulated HDAC1, HDAC2, HDAC6, increased histone H3 acetylation and HAT activity. Moreover, 20(s)-Rh2 inhibited the growth of human leukemia xenograft tumors in vivo. 20(s)-Rh2 inhibited the proliferation of K562 and KG1-α cell by reducing the expression and activity of HDACs, increasing histone acetylation, and regulating key proteins in the downstream signaling pathways. Therefore, 20(s)-Rh2 could become a potential natural HDACi for chemotherapy of leukemia. •20(s)-Rh2 can inhibit the proliferation in K562 and KG1-α cells.•20(s)-Rh2 reduce the expression and activity of HDACs, increase histone acetylation.•20(s)-Rh2 inhibited the growth of human leukemia xenograft tumors in vivo.•20(s)-Rh2 is a natural histone deacetylase inhibit for chemotherapy of leukemia.