Details

Autor(en) / Beteiligte

• van Beuningen, Sam F.B.
• Will, Lena
• Harterink, Martin
• Chazeau, Anaël
• van Battum, Eljo Y.
• Frias, Cátia P.
• Franker, Mariella A.M.
• Katrukha, Eugene A.
• Stucchi, Riccardo
• Vocking, Karin
• Antunes, Ana T.
• Slenders, Lotte
• Doulkeridou, Sofia
• Sillevis Smitt, Peter
• Altelaar, A.F. Maarten
• Post, Jan A.
• Akhmanova, Anna
• Pasterkamp, R. Jeroen
• Kapitein, Lukas C.
• de Graaff, Esther
• Hoogenraad, Casper C.

Titel

TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays

Ist Teil von

• Neuron (Cambridge, Mass.), 2015-12, Vol.88 (6), p.1208-1226

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2015

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Axon formation, the initial step in establishing neuronal polarity, critically depends on local microtubule reorganization and is characterized by the formation of parallel microtubule bundles. How uniform microtubule polarity is achieved during axonal development remains an outstanding question. Here, we show that the tripartite motif containing (TRIM) protein TRIM46 plays an instructive role in the initial polarization of neuronal cells. TRIM46 is specifically localized to the newly specified axon and, at later stages, partly overlaps with the axon initial segment (AIS). TRIM46 specifically forms closely spaced parallel microtubule bundles oriented with their plus-end out. Without TRIM46, all neurites have a dendrite-like mixed microtubule organization resulting in Tau missorting and altered cargo trafficking. By forming uniform microtubule bundles in the axon, TRIM46 is required for neuronal polarity and axon specification in vitro and in vivo. Thus, TRIM46 defines a unique axonal cytoskeletal compartment for regulating microtubule organization during neuronal development. •TRIM46 specifically localizes to the proximal axon•TRIM46 forms closely spaced, parallel microtubule fascicles•TRIM46 is needed for uniform axonal microtubule orientation•TRIM46 is required for axon specification and neuronal polarity in vitro and in vivo van Beuningen et al. show that tripartite motif-containing protein TRIM46 is localized to the proximal axon and forms closely spaced parallel microtubule arrays. Functional analysis demonstrates the importance of uniform axonal microtubule bundles for neuronal polarity and axon specification.