Details

Autor(en) / Beteiligte

• Höllerhage, Matthias
• Rösler, Thomas W.
• Berjas, Magda
• Luo, Rensheng
• Tran, Kevin
• Richards, Kristy M.
• Sabaa-Srur, Armando U.
• Maia, José Guilherme S.
• Moraes, Maria Rosa de
• Godoy, Helena T.
• Höglinger, Günter U.
• Smith, Robert E.

Titel

Neurotoxicity of Dietary Supplements from Annonaceae Species

Ist Teil von

• International journal of toxicology, 2015-11, Vol.34 (6), p.543-550

Ort / Verlag

Los Angeles, CA: SAGE Publications

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Dietary supplements containing plant materials of Annonaceae species (Annona muricata L., A. squamosa L., A. mucosa JACQ., A. squamosa × cherimola Mabb.) were extracted by hot, pressurized ethyl acetate and analyzed for their effect in vitro on Lund human mesencephalic neurons. Cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell death was determined by lactate dehydrogenase levels. Three supplements strongly decreased the cell viability at extract concentrations of 1 µg/mL, of which 1 decreased cell viability at 0.1 µg/µL. Also, strong neuronal toxicities of these supplements were found. Cell death was observed at concentrations of 10 µg/mL. The degree of toxicity was comparable to the ones found in Annonaceous fruit extracts. Two fruit pulps of Annonaceae (A. muricata and A. squamosa) showed a reduction in cell viability at lower concentrations. The fruit pulp extract of A. muricata revealed the strongest neurotoxic effect, with 67% cell death at a concentration of 1 µg/mL. A high reduction in cell viability coupled with pronounced cell death was found at 0.1 µg/mL for an Annonaceous seed extract. These results demonstrate that the intake of dietary supplements containing plant material from Annonaceae may be hazardous to health in terms of neurotoxicity.