Details

Autor(en) / Beteiligte

• Xu, Ling
• Lu, YuHong
• Lai, Jing
• Yu, Wei
• Zhang, YiKai
• Jin, ZhenYi
• Xu, Yan
• Chen, Jie
• Zha, XianFeng
• Chen, ShaoHua
• Yang, LiJian
• Li, YangQiu

Titel

Characteristics of the TCR Vβ repertoire in imatinib-resistant chronic myeloid leukemia patients with ABL mutations

Ist Teil von

• Science China. Life sciences, 2015-12, Vol.58 (12), p.1276-1281

Ort / Verlag

Beijing: Science China Press

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Diversity in the T cell receptor（TCR） repertoire provides a miniature defense ability for the T cell immune system that may be related to tumor initiation and progression. Understanding the T cell immune status of leukemia patients is critical for establishing specific immunotherapies. Previous studies have reported abnormal TCR repertoires and clonally expanded TCR V？ T cells in chronic myeloid leukemia in chronic phase（CP-CML）. In this study, we investigated the distribution and clonality of the TCR V？ repertoire in 4 cases with imatinib-resistant CML in blast crisis（BC-CML） with abelson murine leukemia viral oncogene homolog 1（ABL1） kinase domain mutations（KDMs）. Examination of TCR V？ expression and clonality was performed by reverse transcription-polymerase chain reaction（RT-PCR） and Gene Scan analysis. Significantly skewed TCR V？ repertoires were observed in BC-CML patients with different KDMs, and 4 to 8 oligoclonally expanded TCR V？ subfamilies could be identified in each sample. Intriguingly, a relatively highly expanded V？9 clone with the same length as complementarity-determining region 3（CDR3）（139 bp） was found in all three CML patients in lymphoid blast crisis（LBC-CML） who had different KDMs, but the clone was not detected in the only CML patient in myeloid blast crisis（MBC-CML）. In conclusion, restricted TCR V？ repertoire expression and decreased clone complexity was a general phenomenon observed in the BC-CML patients with different KDMs, indicating the T-cell immunodeficiency of these patients. In addition, clonally expanded V？9 T cell clones may indicate a specific immune response to leukemia-associated antigens in LBC-CML patients.