Details

Autor(en) / Beteiligte

• Hu, Fanghao
• Chen, Zhixing
• Zhang, Luyuan
• Shen, Yihui
• Wei, Lu
• Min, Wei

Titel

Vibrational Imaging of Glucose Uptake Activity in Live Cells and Tissues by Stimulated Raman Scattering

Ist Teil von

• Angewandte Chemie, 2015-08, Vol.127 (34), p.9959-9963

Ort / Verlag

Weinheim: WILEY-VCH Verlag

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Wiley Online Library

Beschreibungen/Notizen

• Glucose is a ubiquitous energy source for most living organisms. Its uptake activity closely reflects cellular metabolic demand in various physiopathological conditions. Extensive efforts have been made to specifically image glucose uptake, such as with positron emission tomography, magnetic resonance imaging, and fluorescence microscopy, but all have limitations. A new platform to visualize glucose uptake activity in live cells and tissues is presented that involves performing stimulated Raman scattering on a novel glucose analogue labeled with a small alkyne moiety. Cancer cells with differing metabolic activities can be distinguished. Heterogeneous uptake patterns are observed with clear cell–cell variations in tumor xenograft tissues, neuronal culture, and mouse brain tissues. By offering the distinct advantage of optical resolution but without the undesirable influence of fluorophores, this method will facilitate the study of energy demands of living systems with subcellular resolution. Eine Schwingungssonde wurde entwickelt, um mit subzellulärer Auflösung die Glucoseaufnahme‐Aktivität in lebenden Zellen und Geweben durch Mikroskopie auf Basis stimulierter Raman‐Streuung (SRS) zu visualisieren. In neuronalen Kulturen und Tumorgeweben wurden heterogene Aufnahmemuster beobachtet, was interessante Perspektiven für das Studium des Energiebedarfs im Gehirn und in Tumoren eröffnet.