Details

Autor(en) / Beteiligte

• Pesti, Miklós
• Gazdag, Zoltán
• Belágyi, József

Titel

In vivo interaction of trivalent chromium with yeast plasma membrane, as revealed by EPR spectroscopy

Ist Teil von

• FEMS microbiology letters, 2000-01, Vol.182 (2), p.375-380

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2000

Quelle

Wiley Online Library

Beschreibungen/Notizen

• The in vivo effects of CrCl 3 on an ergosterol-producing 33 erg + strain of the eukaryotic yeast Candida albicans, and on its ergosterol-deficient erg −2 mutant, were studied by using electron paramagnetic resonance spectroscopy. A 5-doxylstearic acid spin probe was applied to label the membranes. The absence of ergosterol, an increased accumulation of Δ 8 sterols, a decreased fatty acid chainlength and a lower proportion of unsaturated fatty acids of the erg −2 mutant resulted in a higher membrane rigidity and an increased sensitivity to Cr(III) than those of the parental 33 erg + strain. Cr(III) significantly increased the fluidity of the spin labelled membranes, this being more pronounced for the erg −2 mutant. The break in the slopes measured for the erg −2 mutant was decreased (ΔAT∼4°C) from 17 to 13°C. Cr(III) treatment for 10 h caused a loss of metabolites adsorbing at 260 nm: this loss was 40% for 33 erg + and 60% for erg −2 . This decriptification process might be the main cause of growth inhibition and cell killing by the impermeable Cr(III) ions.