Details

Autor(en) / Beteiligte

• Gao, Shumei
• Zhao, Mingxing
• Chen, Yang
• Yu, Meijuan
• Ruan, Wenquan

Titel

Tolerance response to in situ ammonia stress in a pilot-scale anaerobic digestion reactor for alleviating ammonia inhibition

Ist Teil von

• Bioresource technology, 2015-12, Vol.198, p.372-379

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• •Stepwise in situ ammonia-tolerance acclimation was practical and successful.•Anaerobic reactor worked well at an average ammonia concentration of 4275mg/L.•Specific CoF420 content was enhanced with increasing in situ ammonia stress.•Microbial community shift from AM to HM was an in situ response to ammonia stress. The anaerobic digestion (AD) of protein-rich substrates is generally inhibited by ammonia. In this study, ammonia-tolerant acclimation was exposed to a stepwise in situ ammonia stress during the continuous AD of solid residual kitchen waste by using a continuous stirred tank reactor with a 50L active volume. The reactor worked well during the acclimation process, with an average daily biogas production of 58L/d, an effluent soluble chemical oxygen demand of 7238mg/L, a volatile fatty acid (VFA) content of 578mg/L, and a VFA/alkalinity ratio of less than 0.4. Moreover, ammonia stress enhanced the activity of Coenzyme F420. The results of high-throughput 16S rDNA sequencing showed that ammonia stress increased the relative abundance of Firmicutes bacteria and hydrogenotrophic methanogens but decreased the abundance of acetotrophic methanogens. This microbial community shift was proposed to be an in situ response strategy for ammonia stress adaptation.