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Immunoblot and radioallergosorbent test inhibition studies of allergenic cross-reactivity of the predatory mite Amblyseius cucumeris with the house dust mite Dermatophagoides pteronyssinus
Ist Teil von
Annals of allergy, asthma, & immunology, 2004-09, Vol.93 (3), p.281-287
Ort / Verlag
McLean, VA: Elsevier Inc
Erscheinungsjahr
2004
Quelle
MEDLINE
Beschreibungen/Notizen
In 1999, an extensive study among bell pepper growers showed that a predatory mite,
Amblyseius cucumeris, is a potentially relevant source of occupational allergens because 23% of the population had positive skin prick test reactions.
To investigate whether cross-reactivity between
A cucumeris and
Dermatophagoides pteronyssinus is responsible for the cosensitization to both mite species found in 58.7% of
A cucumeris-sensitized greenhouse workers.
Fifteen serum samples from greenhouse workers with work-related inhalant allergy and a positive radioallergosorbent test (RAST) reaction to
A cucumeris or
D pteronyssinus were selected for immunoblot analysis using extracts of both mites. A subselection (n = 5) was used for RAST and immunoblot inhibition to investigate potential cross-reactivity.
On immunoblot, 2 distinct patterns were observed: one pattern showed common protein bands in
A cucumeris and
D pteronyssinus blots suggestive of cross-reactivity between
A cucumeris and
D pteronyssinus and the other pattern showed no shared protein bands.
Dermatophagoides pteronyssinus RAST inhibition with
A cucumeris extract was low in 4 serum samples (<25% inhibition) and nearly absent in 1 serum sample;
A cucumeris RAST inhibition with
D pteronyssinus extract was high in 1 serum sample (75% inhibition), low in 2 serum samples (35% and <15% inhibition), and absent in 2 serum samples. These results were confirmed by immunoblot inhibition experiments.
Amblyseius cucumeris. a new occupational allergen, has species-specific antigens and common antigens that are cross-reactive with the house dust mite
D pteronyssinus.