Details

Autor(en) / Beteiligte

• de Jong, Nicolette W.
• Groenewoud, Giséle C.M.
• van Ree, Ronald
• van Leeuwen, Astrid
• Vermeulen, Ab M.
• van Toorenenbergen, Albert W.
• de Groot, Hans
• van Wijk, Roy G.

Titel

Immunoblot and radioallergosorbent test inhibition studies of allergenic cross-reactivity of the predatory mite Amblyseius cucumeris with the house dust mite Dermatophagoides pteronyssinus

Ist Teil von

• Annals of allergy, asthma, & immunology, 2004-09, Vol.93 (3), p.281-287

Ort / Verlag

McLean, VA: Elsevier Inc

Erscheinungsjahr

2004

Quelle

MEDLINE

Beschreibungen/Notizen

• In 1999, an extensive study among bell pepper growers showed that a predatory mite, Amblyseius cucumeris, is a potentially relevant source of occupational allergens because 23% of the population had positive skin prick test reactions. To investigate whether cross-reactivity between A cucumeris and Dermatophagoides pteronyssinus is responsible for the cosensitization to both mite species found in 58.7% of A cucumeris-sensitized greenhouse workers. Fifteen serum samples from greenhouse workers with work-related inhalant allergy and a positive radioallergosorbent test (RAST) reaction to A cucumeris or D pteronyssinus were selected for immunoblot analysis using extracts of both mites. A subselection (n = 5) was used for RAST and immunoblot inhibition to investigate potential cross-reactivity. On immunoblot, 2 distinct patterns were observed: one pattern showed common protein bands in A cucumeris and D pteronyssinus blots suggestive of cross-reactivity between A cucumeris and D pteronyssinus and the other pattern showed no shared protein bands. Dermatophagoides pteronyssinus RAST inhibition with A cucumeris extract was low in 4 serum samples (<25% inhibition) and nearly absent in 1 serum sample; A cucumeris RAST inhibition with D pteronyssinus extract was high in 1 serum sample (75% inhibition), low in 2 serum samples (35% and <15% inhibition), and absent in 2 serum samples. These results were confirmed by immunoblot inhibition experiments. Amblyseius cucumeris. a new occupational allergen, has species-specific antigens and common antigens that are cross-reactive with the house dust mite D pteronyssinus.